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Attempts at in vitro cultivation of Pneumocystis carinii from human bronchoalveolar lavage fluids without feeder cells.

作者信息

Le Marchand-Auffret B, Polianski J, Roux P

机构信息

Service de Parasitologie, CHU Saint-Antoine, Paris, France.

出版信息

J Protozool. 1991 Nov-Dec;38(6):116S-117S.

PMID:1818129
Abstract

We attempted to cultivate Pneumocystis carinii obtained from two bronchoalveolar lavage fluids of AIDS patients with P. carinii pneumonia, in a system wherein cysteine and 2-mercaptoethanol were substituted for the feeder cells. The presence of P. carinii cysts was monitored for 11 days under conditions of continuous culture. Moderate increase in cyst forms was observed until day 11. Further study with this system would be required to determine if the observed increase in cyst numbers is reproducible and whether the cyst form is a response to adverse in vitro conditions or is a manifestation of growth.

摘要

相似文献

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J Protozool. 1991 Nov-Dec;38(6):116S-117S.
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