真菌荧光法及基因系统荧光抗体法的改良，用于检测支气管肺泡灌洗标本中的卡氏肺孢子虫。
Modification of the fungi-fluor and the genetic systems fluorescent antibody methods for detection of Pneumocystis carinii in bronchoalveolar lavage specimens.
作者信息
Stager C E, Fraire A E, Kim H S, Estrada R, Davis J R, Greenberg D, Guy E S, Guntupalli K K, McBride R A
机构信息
Department of Pathology, Baylor College of Medicine, Houston, Tex.
出版信息
Arch Pathol Lab Med. 1995 Feb;119(2):142-7.
Four hundred forty-five bronchoalveolar lavage specimens from patients with the human immunodeficiency virus were preserved in Saccomano's fixative and stained for Pneumocystis carinii cysts by a modified method with Fungi-Fluor Solution A (Polysciences, Warrington, Pa) and the Genetic Systems Pneumocystis carinii Immunofluorescence Antibody (Genetic Systems Corp, Seattle, Wash). The majority of patients had been treated for suspected P carinii pneumonia for a few days prior to collection of bronchoalveolar lavage specimens. P carinii cysts were detected in 194 (43.6%) specimens. Both stains identified P carinii in 166 (37.3%) specimens and were negative in 251 (56.4%), yielding a concordance rate of 93.7%. P carinii cysts were detected in 25 (5.6%) specimens by the Genetic Systems stain only, and in 3 (0.7%) specimens by the Fungi-Fluor stain only. The sensitivity for detecting cysts of P carinii was significantly greater with the Genetic Systems stain (P < .01).
将445份来自人类免疫缺陷病毒患者的支气管肺泡灌洗标本保存在萨科马诺固定液中，并用改良方法，使用真菌荧光溶液A（Polysciences公司，宾夕法尼亚州沃灵顿）和遗传系统卡氏肺孢子虫免疫荧光抗体（遗传系统公司，华盛顿州西雅图）对卡氏肺孢子虫囊肿进行染色。大多数患者在采集支气管肺泡灌洗标本前几天已因疑似卡氏肺孢子虫肺炎接受治疗。在194份（43.6%）标本中检测到卡氏肺孢子虫囊肿。两种染色方法在166份（37.3%）标本中均鉴定出卡氏肺孢子虫，在251份（56.4%）标本中均为阴性，一致性率为93.7%。仅用遗传系统染色法在25份（5.6%）标本中检测到卡氏肺孢子虫囊肿，仅用真菌荧光染色法在3份（0.7%）标本中检测到。遗传系统染色法检测卡氏肺孢子虫囊肿的敏感性显著更高（P <.01）。
Zotero 插件