Sterling C R, Gilman R H, Sinclair N A, Cama V, Castillo R, Diaz F
Department of Veterinary Science, University of Arizona, Tucson 85721.
J Protozool. 1991 Nov-Dec;38(6):23S-25S.
To test the hypothesis that breast milk of nursing mothers may afford children protection against cryptosporidiosis, a prospective cohort study was carried out in the young peoples' community of San Juan de Miraflores near Lima, Peru. Mothers and newborn children were sorted into cohort groups based on the mothers' breast milk antibody response to Cryptosporidium sporozoites using an antibody-capture enzyme-linked immunosorbent assay to detect parasite-specific immunoglobulin A. Children were monitored for Cryptosporidium infection using an indirect immunofluorescence assay. Of 211 mothers enrolled in the study, 39 (18.5%) had high breast milk antibody titers, 107 (50.7%) had medium titers, and 65 (30.8%) had low titers. Sixty-one episodes of Cryptosporidium infection were detected in 50 children of these mothers. Eleven (22%) had mothers in the high antibody titer group, 20 (40%) had mothers in the medium titer group, and 19 (38%) had mothers in the low titer group. The prevalence of infection within children of each group was 0.17, 0.19 and 0.38 respectively. There was no significant difference in the prevalence or duration of infection among children of the different groups. The data does not support the notion that there is protection from Cryptosporidium infection afforded children whose mothers have demonstrable breast milk antibodies against the parasite.
为验证哺乳母亲的母乳可为儿童提供隐孢子虫病防护这一假说，在秘鲁利马附近的圣胡安德米拉弗洛雷斯青年社区开展了一项前瞻性队列研究。根据母亲母乳对隐孢子虫子孢子的抗体反应，使用抗体捕获酶联免疫吸附测定法检测寄生虫特异性免疫球蛋白A，将母亲和新生儿分为队列组。使用间接免疫荧光测定法监测儿童是否感染隐孢子虫。在参与研究的211名母亲中，39名（18.5%）母乳抗体滴度高，107名（50.7%）滴度中等，65名（30.8%）滴度低。在这些母亲的50名儿童中检测到61次隐孢子虫感染发作。11名（22%）儿童的母亲在高抗体滴度组，20名（40%）儿童的母亲在中等滴度组，19名（38%）儿童的母亲在低滴度组。每组儿童的感染率分别为0.17、0.19和0.38。不同组儿童的感染率或感染持续时间没有显著差异。数据不支持母亲母乳中具有针对该寄生虫的可检测抗体可为儿童提供隐孢子虫感染防护这一观点。
