Sicard Ophélie, Loudet Olivier, Keurentjes Joost J B, Candresse Thierry, Le Gall Olivier, Revers Frédéric, Decroocq Véronique
UMR Génomique, Diversité et Pouvoir Pathogène, Institut National de la Recherche Agronomique-Université Bordeaux 2, IBVM, , BP81, 33883 Villenave d'Ornon, France.
Mol Plant Microbe Interact. 2008 Feb;21(2):198-207. doi: 10.1094/MPMI-21-2-0198.
In compatible interactions between plants and viruses that result in systemic infection, symptom development is a major phenotypic trait. However, host determinants governing this trait are mostly unknown, and the mechanisms underlying it are still poorly understood. In a previous study on the Arabidopsis thaliana-Plum pox virus (PPV) pathosystem, we showed a large degree of variation in symptom development among susceptible accessions. In particular, Cvi-1 (Cape Verde islands) accumulates viral particules but remains symptomless, Col-0 (Columbia) sometimes shows weak symptoms compared with Ler (Landsberg erecta), which always shows severe symptoms. Genetic analyses of Col x Ler and Cvi x Ler F2 and recombinant inbred line (RIL) populations suggested that symptom development as well as viral accumulation traits are polygenic and quantitative. Three of the symptom quantitative trait loci (QTL) identified could be confirmed in near-isogenic lines, including PSI1 (PPV symptom induction 1), which was identified on the distal part of chromosome 1 in both RIL populations. With respect to viral accumulation, several factors have been detected and, interestingly, in the Col x Ler population, two out of three viral accumulation QTL colocalized with loci controlling symptom development, although correlation analysis showed weak linearity between symptom severity and virus accumulation. In addition, in the Cvi x Ler RIL population, a digenic recessive determinant controlling PPV infection was identified.
在导致系统感染的植物与病毒的兼容互作中，症状发展是一个主要的表型特征。然而，控制这一特征的宿主决定因素大多未知，其潜在机制仍知之甚少。在之前关于拟南芥-李痘病毒（PPV）病理系统的研究中，我们发现易感材料在症状发展方面存在很大差异。特别是，Cvi-1（佛得角群岛）积累病毒颗粒但无症状，Col-0（哥伦比亚）与总是表现出严重症状的Ler（兰茨贝格直立型）相比，有时症状较弱。对Col×Ler和Cvi×Ler F2及重组自交系（RIL）群体的遗传分析表明，症状发展以及病毒积累特征是多基因且定量的。在近等基因系中可以确认所鉴定的三个症状数量性状位点（QTL），包括PSI1（PPV症状诱导1），其在两个RIL群体的第1号染色体远端被鉴定出来。关于病毒积累，已经检测到几个因素，有趣的是，在Col×Ler群体中，三个病毒积累QTL中有两个与控制症状发展的位点共定位，尽管相关性分析显示症状严重程度与病毒积累之间的线性关系较弱。此外，在Cvi×Ler RIL群体中，鉴定出一个控制PPV感染的双基因隐性决定因素。
