INRA, UMR 1332 de Biologie du fruit et Pathologie, Villenave d'Ornon, F-33140, France.
Plant Methods. 2014 Jan 22;10(1):2. doi: 10.1186/1746-4811-10-2.
To identify plant genes involved in various key traits, QTL mapping is a powerful approach. This approach is based on the use of mapped molecular markers to identify genomic regions controlling quantitative traits followed by a fine mapping and eventually positional cloning of candidate genes. Mapping technologies using SNP markers are still rather expensive and not feasible in every laboratory. In contrast, microsatellite (also called SSR for Simple Sequence Repeat) markers are technologically less demanding and less costly for any laboratory interested in genetic mapping.
In this study, we present the development and the characterization of a panel of 96 highly polymorphic SSR markers along the Arabidopsis thaliana genome allowing QTL mapping among accessions of the Versailles 24 core collection that covers a high percentage of the A. thaliana genetic diversity. These markers can be used for any QTL mapping analysis involving any of these accessions. We optimized the use of these markers in order to reveal polymorphism using standard PCR conditions and agarose gel electrophoresis. In addition, we showed that the use of only three of these markers allows differentiating all 24 accessions which makes this set of markers a powerful tool to control accession identity or any cross between any of these accessions.
The set of SSR markers developed in this study provides a simple and efficient tool for any laboratory focusing on QTL mapping in A. thaliana and a simple means to control seed stock or crosses between accessions.
为了鉴定参与各种重要性状的植物基因，QTL 作图是一种强大的方法。这种方法基于使用图谱分子标记来鉴定控制数量性状的基因组区域，然后进行精细作图，最终对候选基因进行定位克隆。使用 SNP 标记的作图技术仍然相当昂贵，并且不是每个实验室都可行。相比之下，微卫星（也称为简单序列重复 SSR）标记在技术上要求不高，对于任何有兴趣进行遗传作图的实验室来说成本都较低。
在这项研究中，我们开发并描述了一组 96 个高度多态性的 SSR 标记，这些标记沿着拟南芥基因组排列，允许对覆盖拟南芥遗传多样性很大一部分的凡尔赛 24 核心收集品系进行 QTL 作图。这些标记可用于涉及任何这些品系的任何 QTL 作图分析。我们优化了这些标记的使用，以便在标准 PCR 条件和琼脂糖凝胶电泳下显示多态性。此外，我们还表明，仅使用这三个标记中的三个就可以区分所有 24 个品系，这使得这组标记成为控制品系身份或任何这些品系之间杂交的有力工具。
本研究开发的 SSR 标记集为任何专注于拟南芥 QTL 作图的实验室提供了一种简单有效的工具，也是控制种子库存或品系间杂交的简单手段。
