Rodriguez Milka A, Shen Chengli, Ratner Deena, Paranjape Ramesh S, Kulkarni Smita S, Chatterjee Ramdas, Gupta Phalguni
Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.
AIDS Res Hum Retroviruses. 2007 Nov;23(11):1428-33. doi: 10.1089/aid.2007.0152.
Genetic analysis of HIV-1 sequences circulating in different parts of India have shown that the predominant proportion of HIV-1 subtypes circulating in India is type C and a small fraction are subtypes A, B, E, and CRFs. We sequenced the HIV-1 LTR promoter region of seven subtype C and five subtype A isolates obtained from two major cities in India. Sequence analysis of the complete promoter and TAR regions revealed conserved subtype-specific variability in several major binding sites. Three NF-kappaB sites were present in all subtype C isolates and two isolates contained an insertion in the MFNLP. The transcriptional activity of one of these isolates may have been hindered due to this insertion. Despite the apparent variability between the LTRs we did not observe any significant difference in the transcriptional activity between subtype C and subtype A. To our knowledge, this is the first study characterizing the genetic structure and functional attributes of subtype A LTRs from India.
对在印度不同地区传播的HIV-1序列进行的基因分析表明，在印度传播的HIV-1亚型中，主要比例为C型，一小部分是A、B、E型和循环重组型（CRF）。我们对从印度两个主要城市获得的7株C亚型和5株A亚型分离株的HIV-1长末端重复序列（LTR）启动子区域进行了测序。对完整启动子和反式激活应答元件（TAR）区域的序列分析揭示了几个主要结合位点中保守的亚型特异性变异性。所有C亚型分离株中均存在3个核因子κB（NF-κB）位点，2株分离株在MFNLP中有一个插入。其中一株分离株的转录活性可能由于该插入而受到阻碍。尽管LTR之间存在明显的变异性，但我们未观察到C亚型和A亚型之间的转录活性有任何显著差异。据我们所知，这是第一项对来自印度的A亚型LTR的基因结构和功能特性进行表征的研究。
