Neogi Ujjwal, Sharma Yogeshwar, Sood Vikas, Wanchu Ajay, Banerjea Akhil C
Division of Virology, National Institute of Immunology, New Delhi, India.
AIDS Res Hum Retroviruses. 2010 Dec;26(12):1299-305. doi: 10.1089/aid.2010.0128. Epub 2010 Oct 7.
We genetically characterized the extent of variation in HIV-1 LTR sequences from 11 mother-to-child transmission (MTCT) pairs from HIV-1-infected individuals from North India. Nine pairs were found to be infected with subtype C virus whereas two pairs were infected with subtype B virus. They harbored the characteristic three and two NF-κB sites, respectively. The analysis of intrasubtype divergence between B and C revealed greater diversity with subtype B LTR sequences than subtype C (p < 0.005). Significant evolutionary divergence of subtype C and subtype B was found in NFAT-III (p < 0.000001), NFAT-II (p < 0.0001), and USF (p < 0.005) transcription factor binding sites (TFBS). NF-κB-I, Sp I and II, Ets-I, AP-I and II, and TATA Box TFBS were highly conserved in both the subtypes. An alternate secondary structure of Tar was detected in the VT5 sample due to the point mutation from G to C (position +21) and T to C (position +38).
我们对来自印度北部HIV-1感染者的11对母婴传播（MTCT）样本中的HIV-1长末端重复序列（LTR）的变异程度进行了基因特征分析。发现9对样本感染了C亚型病毒，而2对样本感染了B亚型病毒。它们分别含有特征性的3个和2个核因子κB（NF-κB）位点。对B亚型和C亚型之间的亚型内差异分析显示，B亚型LTR序列的多样性高于C亚型（p < 0.005）。在活化T细胞核因子III（NFAT-III，p < 0.000001）、活化T细胞核因子II（NFAT-II，p < 0.0001）和上游刺激因子（USF，p < 0.005）转录因子结合位点（TFBS）中发现C亚型和B亚型存在显著的进化差异。NF-κB-I、Sp I和II、Ets-I、AP-I和II以及TATA盒TFBS在两种亚型中都高度保守。由于VT5样本中发生了从G到C（位置+21）以及从T到C（位置+38）的点突变，检测到了Tar的一种交替二级结构。
