Dijkink Rory, Le Gac Séverine, Nijhuis Erwin, van den Berg Albert, Vermes István, Poot André, Ohl Claus-Dieter
Physics of Fluids, Faculty of Science and Technology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.
Phys Med Biol. 2008 Jan 21;53(2):375-90. doi: 10.1088/0031-9155/53/2/006. Epub 2007 Dec 28.
Cavitation bubble dynamics close to a rigid surface gives rise to a rapid and transient fluid flow. A single bubble is created with a laser pulse at different stand-off distances from the rigid surface, where the stand-off distance gamma is defined by gamma = h/R(max), with h being the initial distance and R(max) being the maximum bubble radius. When the surface is covered with adherent cells, molecular delivery and cell detachment after single cavitation activity are observed at different locations. We find a maximum of cell detachment at a normalized stand-off distance of gamma approximately 0.65. In contrast, the maximum of the molecular uptake is found when gamma approaches 0. The single cavitation event has only little effect on the viability of cells in the non-detached area. We find apoptosis of cells only very close to the area of detachment and, additionally, the metabolism of the non-detached cells shows no pronounced difference compared to control cells according to an MTS assay. Thus, although the cavitation event is responsible for the detachment of cells, only few of the remaining cells undergo a permanent change.
靠近刚性表面的空化气泡动力学产生快速且瞬态的流体流动。用激光脉冲在距刚性表面不同的间隔距离处产生单个气泡，其中间隔距离γ由γ = h/R(max)定义，h为初始距离，R(max)为气泡最大半径。当表面覆盖有贴壁细胞时，在不同位置观察到单次空化活动后的分子递送和细胞脱离。我们发现在归一化间隔距离γ约为0.65时细胞脱离达到最大值。相比之下，当γ接近0时发现分子摄取达到最大值。单次空化事件对未脱离区域的细胞活力影响很小。我们发现仅在非常靠近脱离区域的细胞发生凋亡，此外，根据MTS测定，未脱离细胞的代谢与对照细胞相比没有明显差异。因此，尽管空化事件导致细胞脱离，但其余细胞中只有少数经历永久性变化。
