Division of Microelectronics, School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
Lab Chip. 2013 Mar 21;13(6):1144-50. doi: 10.1039/c3lc41252k.
This paper demonstrates membrane poration of a single suspension cell due to a fast liquid microjet. The jet is formed during the collapse of a laser induced bubble created at a variable stand-off distance from the target cell. The cell is trapped by a converging structure within a microfluidic chip. The asymmetrical growth and collapse of the cavitation bubble next to the cell lead to the microjetting, which deforms and porates the cell membrane. In the experiments, the membrane porations of myeloma cells are probed with the uptake of trypan blue. Time-resolved studies of the diffusion of trypan blue show a marked dependency on the bubble dynamics, i.e. the stand-off distance. The penetration length of the dye increases with shorter distances. Numerical simulations of the diffusion process agree with larger pores formed on the cell membrane. This method allows for a fast, repeatable, and localized rupture of membranes of individual cells in suspension.
本文展示了由于快速液体微射流而导致单个悬浮细胞的膜穿孔。射流是在激光诱导的气泡在距靶细胞的可变间距处坍塌时形成的。细胞被微流控芯片内的会聚结构捕获。由于紧邻细胞的空化泡的不对称生长和坍塌，导致微射流使细胞膜变形和穿孔。在实验中，用摄取台盼蓝来探测骨髓瘤细胞的膜穿孔。台盼蓝扩散的时间分辨研究表明，其显著依赖于气泡动力学，即间距。染料的穿透长度随距离的缩短而增加。扩散过程的数值模拟与细胞膜上形成的较大孔相吻合。该方法允许快速、可重复且局部地破坏悬浮单个细胞的细胞膜。
