Bakri Sophie J, Pulido Jose S, Mukherjee Priyabata, Marler Ronald J, Mukhopadhyay Debabrata
Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota, USA.
Retina. 2008 Jan;28(1):147-9. doi: 10.1097/IAE.0b013e3180dc9360.
To evaluate the retinal toxicity of intravitreal nanogold, a novel antiangiogenic and long-term delivery agent.
One eye of each of 16 Dutch-belted rabbits was injected with intravitreal nanogold; the other eye served as a control. Eight rabbits received a dose of 67 micromol/0.1 mL of nanogold intravitreally into 1 eye; the other 8 rabbits received a dose of 670 micromol/0.1 mL of nanogold intravitreally into 1 eye. Eight rabbits were killed at 1 week, and eight were killed at 1 month; both eyes of each rabbit were enucleated. The eyes were fixed with 2% paraformaldehyde and sectioned for histologic examination.
In all injected and control eyes, there was mild vacuolization in the inner plexiform and ganglion cell layers. The retina and retinal pigment epithelium were otherwise histologically normal.
Intravitreal nanogold at concentrations of 67 micromol/0.1 mL and 670 micromol/0.1 mL showed no signs of retinal or optic nerve toxicity by light microscopy during histologic examination at 1 month.
评估新型抗血管生成及长效递送剂玻璃体内纳米金的视网膜毒性。
16只荷兰带兔，每只兔的一只眼玻璃体内注射纳米金；另一只眼作为对照。8只兔的一只眼玻璃体内注射剂量为67微摩尔/0.1毫升的纳米金；另外8只兔的一只眼玻璃体内注射剂量为670微摩尔/0.1毫升的纳米金。8只兔在1周时处死，8只兔在1个月时处死；每只兔的双眼均摘除。眼睛用2%多聚甲醛固定并切片进行组织学检查。
在所有注射纳米金的眼和对照眼中，内网状层和神经节细胞层有轻度空泡化。视网膜和视网膜色素上皮在组织学上其他方面正常。
在组织学检查的1个月时，通过光学显微镜观察，浓度为67微摩尔/0.1毫升和670微摩尔/0.1毫升的玻璃体内纳米金未显示出视网膜或视神经毒性迹象。
