Suemori I, Yoshitake J
Tohoku J Exp Med. 1976 Jun;119(2):123-33. doi: 10.1620/tjem.119.123.
Antishock effect of N6, O2-dibutyryl cyclic AMP (DBcAMP) Was investigated in rats subjected to Noble-Collip drum trauma and compared with effects of hydrocortisone and Trasylol. Results obtained are as follows. 1)Hydrocortisone and Trasylol administered 1 hr before initiating drumming improved the survival rate from traumatic shock with concomitant reducton of levels of acid phosphatase and beta-glucuronidase in circulating blood. DBcAMP administered i.p. immediately after trauma also improved the survival rate to the same extent as did Traylol or hydrocortisone, while no inhibitory effects were observed on acid phosphatase and beta-glucuronidase. 2)The rectal temperature fell significantly after suffering trauma, and the rats with greater fall in rectal temperature had poorer chance for survival. The fall in rectal temperature was considerably prevented by DBcAMP in a dose of 0.5 mg/100 g body weight (b.w.). 3)DBcAMP induced a rise in plasma insulin level (IRI) and insulin/glucose ratio (I/G) in shock rats, and the elevation in blood lactate/pyruvate ratio (L/P) and excess lactate otherwise observed after trauma were satisfactorily prevented by DBcAMP administration. It is concluded that the antichock effects of DBcAMP primarily resulted from improvements of the intracellular metabolism induced by its easy passage through the cell membrane and its cAMP like action, while any preventive action was not observed against elevation of lysosomal enzymes in the circulating blood.
研究了 N6,O2 - 二丁酰环磷酸腺苷（DBcAMP）对 Noble - Collip 鼓式创伤大鼠的抗休克作用，并与氢化可的松和抑肽酶的作用进行了比较。得到的结果如下。1）在开始击鼓前 1 小时给予氢化可的松和抑肽酶可提高创伤性休克的存活率，同时降低循环血液中酸性磷酸酶和β - 葡萄糖醛酸酶的水平。创伤后立即腹腔注射 DBcAMP 也能将存活率提高到与抑肽酶或氢化可的松相同的程度，而对酸性磷酸酶和β - 葡萄糖醛酸酶没有抑制作用。2）创伤后直肠温度显著下降，直肠温度下降幅度较大的大鼠存活机会较差。剂量为 0.5mg/100g 体重（b.w.）的 DBcAMP 可显著防止直肠温度下降。3）DBcAMP 可使休克大鼠血浆胰岛素水平（IRI）和胰岛素/葡萄糖比值（I/G）升高，并且给予 DBcAMP 可令人满意地防止创伤后原本观察到的血乳酸/丙酮酸比值（L/P）升高和乳酸过量。得出的结论是，DBcAMP 的抗休克作用主要源于其易于穿过细胞膜及其类似 cAMP 的作用所诱导的细胞内代谢改善，而未观察到对循环血液中溶酶体酶升高的任何预防作用。
