用于线粒体呼吸复合体的高通量蓝色天然聚丙烯酰胺凝胶电泳
High-Throughput BN-PAGE for Mitochondrial Respiratory Complexes.
作者信息
Vincis Pereira Sanglard Lilian, Colas des Francs-Small Catherine
机构信息
ARC Centre of Excellence for Plant Energy Biology, The University of Western Australia, Crawley, Australia.
出版信息
Methods Mol Biol. 2022;2363:111-119. doi: 10.1007/978-1-0716-1653-6_10.
Blue native electrophoresis (BN-PAGE) is a highly resolutive method suited to the study of high molecular weight protein complexes between 100 and >3000 kDa. One of the drawbacks of this method is that it is very time-consuming and requires high quantities of purified organelles. Here we describe a high throughput BN-PAGE method allowing to screen libraries of plants potentially altered in respiratory metabolism.
蓝色非变性聚丙烯酰胺凝胶电泳（BN-PAGE）是一种分辨率很高的方法，适用于研究分子量在100至3000 kDa以上的高分子量蛋白质复合物。该方法的缺点之一是非常耗时，并且需要大量纯化的细胞器。在此，我们描述了一种高通量BN-PAGE方法，可用于筛选呼吸代谢可能发生改变的植物文库。
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