Scott-Fordsmand Janeck J, Maraldo Kristine, van den Brink Paul J
Department of Terrestrial Ecology, National Environmental Research Institute, University of Aarhus, Vejlsøvej 25, DK-8600 Silkeborg, Denmark.
Environ Int. 2008 May;34(4):524-30. doi: 10.1016/j.envint.2007.11.008. Epub 2008 Jan 14.
A gnotobiotic multi-species study was designed to consist of a food-web of soil-dwelling animals. The food-web was exposed to five concentrations copper (Cu) spiked soil for three exposure durations i.e. 28, 56 and 84 days. Based on multivariate analysis the food-web was significantly affected by Cu exposure at and above 300 mg Cu kg(-1) soil (lowest tested concentration). The number of animals present in the 2500 mg Cu kg(-1) (highest tested concentration) was at all sampling occasions below the starting point level. Based on analysis of the individual species the lowest 10% effect concentration (EC10) observed was 50 mg Cu kg(-1) soil, for Enchytraeus crypticus. Using the EC10 for the individual species the HC5 (Hazard Concentration at the 5% level) was estimated to be between 25 and 36 mg Cu kg(-1) soil, depending on the exposure duration. A similar experiment but using a reduced design was performed employing soil contaminated with Cu in the field more than 80 years ago. The trend in the field-contaminated soil was similar to that observed for the spiked soil.
设计了一项无菌多物种研究，该研究由一个土壤动物食物网组成。该食物网暴露于添加了五种浓度铜（Cu）的土壤中，暴露持续时间为28天、56天和84天。基于多变量分析，当土壤中铜含量达到及高于300毫克/千克（最低测试浓度）时，食物网受到铜暴露的显著影响。在所有采样时刻，2500毫克/千克（最高测试浓度）土壤中的动物数量均低于起始水平。基于对单个物种的分析，观察到的最低10%效应浓度（EC10）为50毫克/千克土壤，针对的是隐尾蚓。根据单个物种的EC10，估计HC5（5%水平的危害浓度）在25至36毫克/千克土壤之间，具体取决于暴露持续时间。80多年前在野外进行了一项类似的实验，但采用了简化设计，使用受铜污染的土壤。野外污染土壤中的趋势与添加铜土壤中观察到的趋势相似。
