Abdelalim Essam Mohamed, Masuda Chiaki, Tooyama Ikuo
Molecular Neuroscience Research Center, Shiga University of Medical Science, Seta, Otsu, Shiga 520-2192, Japan.
Peptides. 2008 Apr;29(4):622-8. doi: 10.1016/j.peptides.2007.11.021. Epub 2007 Dec 5.
Recently, the natriuretic peptides were detected in the cholinergic and dopaminergic amacrine cells of the retina. We performed immunofluorescence labeling of rat retinal sections to examine the immunoreactivity of natriuretic peptide-activated guanylate cyclases (NPR-A and NPR-B) in the rat retina, in particular whether they were localized to dopaminergic and cholinergic amacrine cells. NPR-A and NPR-B immunoreactivity was detected in several layers of the retina including amacrine cells. In amacrine cells, both NPR-A and NPR-B were co-localized with tyrosine hydroxylase, a marker of dopaminergic cells. NPR-B, but not NPR-A, was localized to amacrine cells expressing choline acetyltransferase (ChAT), a marker of cholinergic cells. These findings suggest that natriuretic peptides have different regulatory systems in dopaminergic and cholinergic amacrine cells in rat retina.
最近，在视网膜的胆碱能和多巴胺能无长突细胞中检测到了利钠肽。我们对大鼠视网膜切片进行免疫荧光标记，以检查利钠肽激活的鸟苷酸环化酶（NPR - A和NPR - B）在大鼠视网膜中的免疫反应性，特别是它们是否定位于多巴胺能和胆碱能无长突细胞。在包括无长突细胞在内的视网膜几层中检测到了NPR - A和NPR - B免疫反应性。在无长突细胞中，NPR - A和NPR - B均与酪氨酸羟化酶共定位，酪氨酸羟化酶是多巴胺能细胞的标志物。NPR - B而非NPR - A定位于表达胆碱乙酰转移酶（ChAT）的无长突细胞，胆碱乙酰转移酶是胆碱能细胞的标志物。这些发现表明，利钠肽在大鼠视网膜的多巴胺能和胆碱能无长突细胞中具有不同的调节系统。
