School of Life Science and Technology, Changchun University of Science and Technology, 7989 Weixing Road, Changchun 130022, China.
Brain Res Bull. 2010 May 31;82(3-4):188-92. doi: 10.1016/j.brainresbull.2010.03.004. Epub 2010 Mar 19.
Natriuretic peptides (NPs) exert their actions through three membrane-bound receptors, which are known as NP receptors (NPRs: NPR-A, NPR-B and NPR-C). In this work we examined the expression of three NPRs in rat retinal ganglion cells (GCs), retrogradely labeled and intracellularly dye-injected, by double immunofluorescence labeling. In vertical sections, almost all GCs, retrogradely labeled by cholera toxin B, were stained by antibodies against the three NPRs. The labeling for three NPRs was observed mainly on the membranes of the somata of GCs, whereas the staining for NPR-A was also seen in the cytoplasm. Moreover, with tangential sections, almost all cells located in the ganglion cell layer were NPR-A, B, C immunoreactive. By combining with intracellular injection of Neurobiotin into GCs in whole mount retinas that enables to identify ON-, OFF- and ON-OFF-types of GCs according to arborization of their dendrites in the inner plexiform layer, we further demonstrated that NPRs were expressed in these major types of GCs.
利钠肽（NPs）通过三种膜结合受体发挥作用，这三种受体被称为 NP 受体（NPRs：NPR-A、NPR-B 和 NPR-C）。在这项工作中，我们通过双重免疫荧光标记检查了逆行标记和细胞内染色注射的大鼠视网膜神经节细胞（GCs）中三种 NPRs 的表达。在垂直切片中，几乎所有用霍乱毒素 B 逆行标记的 GCs 均被三种 NPRs 的抗体染色。三种 NPRs 的标记主要观察到 GCs 体的细胞膜上，而 NPR-A 的染色也可见于细胞质中。此外，用切线切片，几乎所有位于神经节细胞层中的细胞均对 NPR-A、B、C 具有免疫反应性。通过将 Neurobiotin 注入整个视网膜的 GCs 中进行组合，这使得能够根据其树突在神经内丛状层中的分支来识别 ON-、OFF- 和 ON-OFF 型 GCs，我们进一步证明了这些主要类型的 GCs 中表达了 NPRs。
