Nakamura M
Second Department of Surgery, Yamaguchi University School of Medicine, Ube-shi, Japan.
Nihon Geka Hokan. 1991 Jan 1;60(1):3-12.
A new human esophageal cancer cell line (YES-1) was established from a subcutaneous tumor implanted into nude mice, which had been transplanted from a surgical specimen obtained 50-year-old Japanese male patient. This cell line has been maintained for 33 months through 94 passages with stable growth. YES-1 cells are mainly polygonal-to-spindle shaped, have eosinophilic cytoplasm and oval-to-round nuclei with some prominent nucleoli. There are also some cells having clear cytoplasm and round nuclei with prominent nucleoli. The cells proliferate in a pavement-like cell arrangement and show a lack of contact inhibition. The doubling time at the 33rd passage was 35.2 hours. YES-1 cells produce carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC antigen) and tissue polypeptide antigen (TPA) as tumor markers. Chromosome study have shown that the chromosome number ranges from 47 to 54 with a mode of 51. Tumorigenicity has been identified by the development of tumors after the subcutaneous injection of YES-1 cells into nude mice, which were found to be similar to the original tumor on histological examination. Thus, these findings indicate that the YES-1 cell line is available as a new human esophageal cancer cell line which should be useful for various studies.
一种新的人食管癌细胞系（YES-1）是从接种于裸鼠的皮下肿瘤中建立的，该皮下肿瘤源自一名50岁日本男性患者的手术标本移植。该细胞系已通过94代培养维持了33个月，生长稳定。YES-1细胞主要呈多边形至梭形，具有嗜酸性细胞质以及椭圆形至圆形的细胞核，伴有一些明显的核仁。也有一些细胞具有清亮的细胞质和圆形细胞核，核仁明显。这些细胞以铺路石样的细胞排列方式增殖，且表现出缺乏接触抑制。第33代时的倍增时间为35.2小时。YES-1细胞可产生癌胚抗原（CEA）、鳞状细胞癌抗原（SCC抗原）和组织多肽抗原（TPA）作为肿瘤标志物。染色体研究表明，染色体数目范围为47至54条，众数为51条。通过将YES-1细胞皮下注射到裸鼠体内后肿瘤的形成确定了其致瘤性，组织学检查发现这些肿瘤与原始肿瘤相似。因此，这些结果表明YES-1细胞系可作为一种新的人食管癌细胞系，对各种研究应具有实用性。
