Murakami T, Nakamura M, Kusanagi H, Suzuki T
Second Department of Surgery, Yamaguchi University School of Medicine, Ube, Japan.
Nihon Geka Gakkai Zasshi. 1991 Nov;92(11):1563-70.
Three squamous cell carcinoma cell lines, YES-1, YES-2, and YES-3, were established from human esophageal carcinoma. Two cell lines, YES-1 and YES-2, were established from the surgically removed tumor and YES-3 was established from pleural effusion of the pleuritis carcinomatosa by esophageal carcinoma. These three cells not only resembled the original esophageal carcinoma tissue on microscopic examination, but also produced same tumor marker, such as SCC antigen, CEA, TPA, CA125 and CA19-9. However, the secretion patterns of tumor markers were different. The doubling times of YES-1 cells, YES-2 cells and YES-3 cells were 35.2 hours, 22.9 hours, and 61.0 hours, respectively. Chromosomal studies show that chromosomal number of YES-1 cells ranges 47 to 54 with a mode of 51, that of YES-2 cells ranges 53 to 62 with a mode of 60 and that of YES-3 cells ranges 48 to 52 with a mode of 50. These three cells grow both in serum-containing medium and in serum free medium. When YES-1, YES-2, and YES-3 cells were injected into athymic nude mice subcutaneously, they introduced tumors which resembled the original esophageal carcinoma tissue histologically. These 3 cell lines may provide useful models for the study of human esophageal carcinoma.
三种鳞状细胞癌细胞系，YES-1、YES-2和YES-3，是从人食管癌中建立的。其中两种细胞系，YES-1和YES-2，是从手术切除的肿瘤中建立的，而YES-3是从食管癌所致癌性胸膜炎的胸腔积液中建立的。这三种细胞在显微镜检查下不仅与原始食管癌组织相似，而且还产生相同的肿瘤标志物，如鳞状细胞癌抗原、癌胚抗原、组织多肽抗原、糖类抗原125和糖类抗原19-9。然而，肿瘤标志物的分泌模式有所不同。YES-1细胞、YES-2细胞和YES-3细胞的倍增时间分别为35.2小时、22.9小时和61.0小时。染色体研究表明，YES-1细胞的染色体数目在47至54之间，众数为51；YES-2细胞的染色体数目在53至62之间，众数为60；YES-3细胞的染色体数目在48至52之间，众数为50。这三种细胞在含血清培养基和无血清培养基中均能生长。当将YES-1、YES-2和YES-3细胞皮下注射到无胸腺裸鼠体内时，它们会形成组织学上类似于原始食管癌组织的肿瘤。这三种细胞系可能为人类食管癌的研究提供有用的模型。
