Guan Yingfu, Zhang Wei, Deng Maicun, Jin Meifang, Yu Xingju
Marine Bioproducts Engineering Group, Dalian Institute of Chemical and Physics, Chinese Academy of Sciences, Dalian 116023, China.
Biotechnol Lett. 2004 Jul;26(13):1031-5. doi: 10.1023/B:BILE.0000032961.71564.00.
A marine green microalga, Platymonas subcordiformis, photo-synthetically generates H(2) but only transiently at a negligible yield when exposed to light after a period of dark anaerobic incubation. A protonophore uncoupler, carbonyl cyanide m-chlorophenylhrazone (CCCP) significantly increased the yield of H(2) photo-production. CCCP optimally at 15 microM gave 4.9 ml H(2) after 8 h light irradiation in 1 l algal cell culture at 1.8 x 10(6) cells ml(-1). The H(2) yield at 15 microM CCCP was increased by 240-fold when compared to the control. This improvement may be by CCCP disrupting the proton motive force thus facilitating proton transfer across the thylakoidal membrane.
一种海洋绿色微藻，亚心形扁藻，在黑暗厌氧培养一段时间后暴露于光照下，能通过光合作用产生氢气，但产量极低且只是短暂产生。一种质子载体解偶联剂，羰基氰化物间氯苯腙（CCCP）显著提高了光合产氢量。在1升藻细胞培养物（细胞密度为1.8×10⁶个细胞/毫升）中，15微摩尔的CCCP在光照8小时后可产生4.9毫升氢气。与对照组相比，15微摩尔CCCP时的氢气产量提高了240倍。这种改善可能是由于CCCP破坏了质子动力势，从而促进质子跨类囊体膜的转移。
