Berchane N S, Andrews M J, Kerr S, Slater N K H, Jebrail F F
Department of Mechanical Engineering, Texas A&M University, College Station, TX, USA.
J Mater Sci Mater Med. 2008 Apr;19(4):1831-8. doi: 10.1007/s10856-007-3360-7. Epub 2008 Jan 16.
Biological adhesives, natural and synthetic, are of current active interest. These adhesives offer significant advantages over traditional sealant techniques, in particular, they are easier to use, and can play an integral part in the healing mechanism of tissue. Thus, biological adhesives can play a major role in medical applications if they possess adequate mechanical behavior and stability over time. In this work, we report on the method of preparation of bovine serum albumin (BSA) into a biological adhesive. We present quantitative measurements that show the effect of BSA concentration and cross-linker content on the bonding strength of BSA adhesive to wood. A comparison is then made with synthetic poly(glycidyl methacrylate) (PGMA) adhesive, and a commercial cyanoacrylate glue, which was used as a control adhesive. In addition, BSA samples were prepared and characterized for their water content, tensile strength, and elasticity. We show that on dry surface, BSA adhesive exhibits a high bonding strength that is comparable with non-biological commercial cyanoacrylate glues, and synthetic PGMA adhesive. Tensile testing on wet wood showed a slight increase in the bonding strength of BSA adhesive, a considerable decrease in the bonding strength of cyanoacrylate glue, and negligible adhesion of PGMA. Tests performed on BSA samples demonstrate that initial BSA concentration and final water content have a significant effect on the stress-strain behavior of the samples.
天然和合成生物粘合剂目前备受关注。这些粘合剂相较于传统密封技术具有显著优势，特别是它们使用起来更简便，并且能在组织愈合机制中发挥不可或缺的作用。因此，如果生物粘合剂具备足够的力学性能和长期稳定性，那么它们在医疗应用中可发挥重要作用。在这项工作中，我们报告了将牛血清白蛋白（BSA）制备成生物粘合剂的方法。我们给出了定量测量结果，展示了BSA浓度和交联剂含量对BSA粘合剂与木材粘结强度的影响。然后将其与合成聚甲基丙烯酸缩水甘油酯（PGMA）粘合剂以及用作对照粘合剂的市售氰基丙烯酸酯胶水进行比较。此外，还制备了BSA样品，并对其含水量、拉伸强度和弹性进行了表征。我们发现，在干燥表面上，BSA粘合剂表现出与非生物市售氰基丙烯酸酯胶水以及合成PGMA粘合剂相当的高粘结强度。对湿木材进行的拉伸测试表明，BSA粘合剂的粘结强度略有增加，氰基丙烯酸酯胶水的粘结强度大幅下降，而PGMA的粘附力可忽略不计。对BSA样品进行的测试表明，初始BSA浓度和最终含水量对样品的应力 - 应变行为有显著影响。
