Bioengineering Department, Clemson University, Clemson, South Carolina.
Materials Science and Engineering Department, Clemson University, Clemson, South Carolina.
J Biomed Mater Res B Appl Biomater. 2019 May;107(4):1047-1055. doi: 10.1002/jbm.b.34197. Epub 2018 Sep 29.
Tissue adhesive has notable clinical benefits in hernia repair fixation. A novel poloxamine tissue adhesive was previously shown to successfully bond collagen tissue with adequate adhesive strength. In application related to attachment of polypropylene (PP) mesh, the adhesive strength between the mesh and poloxamine hydrogel adhesive is limited by the hydrophobicity of PP monofilaments and lack of covalent bond formation. The purpose of this study was to compare two different surface modifications [bovine serum albumin (BSA) adsorption and poly-glycidyl methacrylate/human serum albumin (PGMA/HSA) grafting] of PP mesh for improving the adhesive strength between poloxamine hydrogel adhesive and PP mesh. The PGMA/HSA surface modification significantly improved the adhesive strength for meshes attached with poloxamine hydrogel tissue adhesive compared with unmodified meshes and meshes modified by BSA adsorption. An area of 1 cm adhesive provided for a maximum adhesive strength of 65-70 kPa for meshes modified by PGMA/HSA, 4-13 kPa for meshes modified by BSA, and 22-45 kPa for unmodified meshes. Optical microscopy and infrared spectroscopy (FTIR) confirmed the improved adhesive strength was achieved through mechanical interlock of the hydrogel tissue adhesive into the PP mesh pores and chemical bonding of the albumin after successful PGMA/HSA grafting onto the PP monofilaments. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1047-1055, 2019.
组织粘合剂在疝修补固定中有显著的临床益处。先前已经证明，一种新型的聚氧乙烯胺组织粘合剂可以成功地将胶原组织粘合在一起，具有足够的粘合强度。在与聚丙烯（PP）网片附着相关的应用中，网片和聚氧乙烯胺水凝胶粘合剂之间的粘合强度受到 PP 单丝的疏水性和缺乏共价键形成的限制。本研究的目的是比较两种不同的表面改性方法[牛血清白蛋白（BSA）吸附和聚缩水甘油甲基丙烯酸酯/人血清白蛋白（PGMA/HSA）接枝]对提高聚氧乙烯胺水凝胶粘合剂和 PP 网片之间的粘合强度的作用。与未改性的网片和经 BSA 吸附改性的网片相比，PGMA/HSA 表面改性显著提高了与聚氧乙烯胺水凝胶组织粘合剂附着的网片的粘合强度。对于经 PGMA/HSA 改性的网片，每平方厘米的粘合面积提供的最大粘合强度为 65-70 kPa，经 BSA 改性的网片为 4-13 kPa，未改性的网片为 22-45 kPa。光学显微镜和红外光谱（FTIR）证实，通过水凝胶组织粘合剂机械地嵌入到 PP 网片的孔隙中以及成功地将 PGMA/HSA 接枝到 PP 单丝上后白蛋白的化学结合，提高了粘合强度。© 2018 年 Wiley 期刊出版公司。J 生物医学材料研究杂志 B：应用生物材料 107B：1047-1055，2019 年。
