Roberts A D, Finnigan W, Kelly P P, Faulkner M, Breitling R, Takano E, Scrutton N S, Blaker J J, Hay S
Department of Chemistry, Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN, UK.
Bio-Active Materials Group, School of Materials, The University of Manchester, Manchester, M1 2PG, UK.
Mater Today Bio. 2020 Jul 10;7:100068. doi: 10.1016/j.mtbio.2020.100068. eCollection 2020 Jun.
Protein-based adhesives could have several advantages over petroleum-derived alternatives, including substantially lower toxicity, smaller environmental footprint, and renewable sourcing. Here, we report that non-covalently crosslinked bovine serum albumin and recombinant spider silk proteins have high adhesive strength on glass (8.53 and 6.28 MPa, respectively) and other transparent substrates. Moreover, the adhesives have high visible transparency and showed no apparent degradation over a period of several months. The mechanism of adhesion was investigated and primarily attributed to dehydration-induced reorganization of protein secondary structure, resulting in the supramolecular association of β-sheets into a densely hydrogen-bonded network.
基于蛋白质的粘合剂相对于石油衍生的替代品可能具有几个优点，包括毒性显著更低、环境足迹更小以及可再生来源。在此，我们报告非共价交联的牛血清白蛋白和重组蜘蛛丝蛋白在玻璃（分别为8.53和6.28兆帕）和其他透明基材上具有高粘合强度。此外，这些粘合剂具有高可见光透明度，并且在几个月的时间内没有明显降解。对粘附机制进行了研究，主要归因于脱水诱导的蛋白质二级结构重组，导致β-折叠超分子缔合成密集的氢键网络。
