Komisarek J, Antkowiak I
Department of Cattle Breeding and Milk Production, Faculty of Animal Breeding and Biology, August Cieszkowski Agricultural University, Wojska Polskiego 71A, 60-625 Poznań, Poland.
Pol J Vet Sci. 2007;10(4):193-7.
The aim of the study was to analyse the relationship between three leptin gene polymorphisms, and indices of reproductive performance--age at first calving, days open, calving interval, number of inseminations per conception, and gestation length in Jersey cattle. A total of 219 cows were tested for the R4C, A59V and C(-963)T mutations using the PCR-RFLP technique. The following allele frequencies were found: R4C--0.80 (C) and 0.20 (T), A59V--0.67 (C) and 0.33 (T), C(-963)T--0.83 (C) and 0.17 (T). The statistically significant correlation (P < or = 0.05) was found only for the A59V mutation. Cows with the TT genotype were characterized by shorter calving interval and time of days open than animals with CT and CC genotype. Also, the number of inseminations per conception was lower in A59V--TT homozygotes compared with two other genotypes. The association analysis did not reveal any significant correlations for both R4C and C(-963)T polymorphisms.
本研究的目的是分析泽西牛的三种瘦素基因多态性与繁殖性能指标（首次产犊年龄、空怀天数、产犊间隔、每次受孕的输精次数和妊娠期长度）之间的关系。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对总共219头奶牛进行了R4C、A59V和C(-963)T突变检测。发现以下等位基因频率：R4C——0.80（C）和0.20（T），A59V——0.67（C）和0.33（T），C(-963)T——0.83（C）和0.17（T）。仅在A59V突变中发现了具有统计学意义的相关性（P≤0.05）。与CT和CC基因型的动物相比，TT基因型的奶牛产犊间隔和空怀天数较短。此外，与其他两种基因型相比，A59V-TT纯合子每次受孕的输精次数也较少。关联分析未发现R4C和C(-963)T多态性有任何显著相关性。
