Jecminkova Katerina, Müller Uwe, Kyselova Jitka, Sztankoova Zuzana, Zavadilova Ludmila, Stipkova Miloslava, Majzlik Ivan
Institute of Animal Science, Prague 10400, Czech Republic.
Faculty of Agrobiology Food and Natural Resources, Czech University of Life Sciences Prague, Prague 16500, Czech Republic.
Asian-Australas J Anim Sci. 2018 Nov;31(11):1721-1728. doi: 10.5713/ajas.17.0900. Epub 2018 Apr 12.
The use of genetic markers can help to enhance reproduction in cattle, which is a very important trait for profitability in dairy production systems. This study evaluated the association between genotypes of leptin (LEP), toll-like receptor 4 (TLR4), and chemokine receptor of interleukin 8 C-X-C motif (CXCR1) genes and fertility traits in Czech Fleckvieh cattle.
Phenotypic data from 786 Czech Fleckvieh cows raised on 5 farms in the Czech Republic were used, along with information from the 1st three parities. To determine genotype, the polymerase chain reaction- restriction fragment length polymorphism method was used.
Except for LEP g.-963C>T, all studied genotype frequencies of single nucleotide polymorphisms (SNPs) were distributed according to the Hardy-Weinberg equilibrium. Two LEP SNPs (g.-963C>T and c.357C>T) were associated with the age at the 1st calving, days open (DO), pregnancy rate after 1st service (PR), and calving interval (CLI). In LEP g.-963C>T the TT genotype heifers firstly calved 24 days earlier than CC genotype and the CT genotype cow showed a tendency for shorter DO and higher PR. In LEP c.357C>T we observed longer CLI and DO period in TT cows. In general, we can propose the TT genotype of g.-963C>T as favorable and the TT genotype of c.357C>T as unfavorable for a cow´s fertility. Heterozygotes in TLR4 c.-226C>G were significantly associated with shorter CLI, and presented a nonsignificant tendency to be associated with higher PR. In CXCR1 c.777 C>G, we did not observe any relationship of this SNP with reproduction.
Overall, the results showed that LEP could be an effective marker for improving reproduction in Czech Fleckvieh cattle. This study also provides novel insights into the relationship between TLR4 and CXCR1 SNPs and reproduction in dual-purpose cattle.
使用遗传标记有助于提高奶牛繁殖力，这是奶牛生产系统盈利能力的一个非常重要的性状。本研究评估了捷克弗莱维赫牛中瘦素（LEP）、Toll样受体4（TLR4）和白细胞介素8 C-X-C基序趋化因子受体（CXCR1）基因的基因型与繁殖性状之间的关联。
使用了来自捷克共和国5个农场饲养的786头捷克弗莱维赫奶牛的表型数据，以及前三个胎次的信息。为了确定基因型，采用了聚合酶链反应-限制性片段长度多态性方法。
除LEP g.-963C>T外，所有研究的单核苷酸多态性（SNP）基因型频率均符合哈迪-温伯格平衡。两个LEP SNP（g.-963C>T和c.357C>T）与首次产犊年龄、空怀天数（DO）、首次输精后的妊娠率（PR）和产犊间隔（CLI）相关。在LEP g.-963C>T中，TT基因型的小母牛比CC基因型的小母牛首次产犊早24天，CT基因型的母牛显示出空怀天数较短和妊娠率较高的趋势。在LEP c.357C>T中，我们观察到TT基因型母牛的产犊间隔和空怀天数较长。总体而言，我们可以提出g.-963C>T的TT基因型对奶牛繁殖力有利，而c.357C>T的TT基因型不利。TLR4 c.-226C>G的杂合子与较短的产犊间隔显著相关，并且与较高的妊娠率有不显著的关联趋势。在CXCR1 c.777 C>G中，我们未观察到该SNP与繁殖之间的任何关系。
总体而言，结果表明LEP可能是改善捷克弗莱维赫牛繁殖力的有效标记。本研究还为TLR4和CXCR1 SNP与兼用型牛繁殖之间的关系提供了新的见解。
