Nomura Yuko, Sugita Yasuo, Yoshida Shiro, Karube Kennosuke, Shimizu Kei, Aoki Ryosuke, Hirose Shinichi, Kikuchi Masahiro, Ohshima Koichi
Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
Pathol Int. 2008 Feb;58(2):98-103. doi: 10.1111/j.1440-1827.2007.02196.x.
The aim of the present study was to estimate the relationship between apoptosis and cell proliferation in histiocytic necrotizing lymphadenitis (HNL). Fifteen patients with HNL were retrospectively analyzed. The patients were divided into three groups according to the proportion of the necrotic area as follows: necrosis (+), necrotic area <25%; necrosis (++), necrotic area 25-50%; and necrosis (+++), necrotic area >50%. Immunohistochemical double staining was performed for CD3 plus caspase-3 and for Ki-67 plus caspase-3 and positive cells were counted in two areas: one without and one with obvious apoptotic features. Most caspase-3-positive cells were also stained for CD3 (area exhibiting obvious apoptotic features: average, 92.3%). Furthermore, various proportions of both Ki-67- and caspase-3-positive cells were detected in all the groups (range, 5-70%). In the area with obvious apoptotic changes, the average percentage of both Ki-67- and caspase-3-positive cells (38.6%) was higher than that in the area without obvious apoptotic features (16.3%). A proportion of cells in HNL undergo proliferation and apoptosis simultaneously, such as neoplastic cells, thereby exhibiting rapid cell cycles.
本研究的目的是评估组织细胞坏死性淋巴结炎（HNL）中细胞凋亡与细胞增殖之间的关系。对15例HNL患者进行回顾性分析。根据坏死面积比例将患者分为三组，如下：坏死（+），坏死面积<25%；坏死（++），坏死面积25 - 50%；坏死（+++），坏死面积>50%。对CD3加半胱天冬酶-3以及Ki-67加半胱天冬酶-3进行免疫组织化学双重染色，并在两个区域对阳性细胞进行计数：一个无明显凋亡特征的区域和一个有明显凋亡特征的区域。大多数半胱天冬酶-3阳性细胞也被CD3染色（表现出明显凋亡特征的区域：平均为92.3%）。此外，在所有组中均检测到不同比例的Ki-67和半胱天冬酶-3阳性细胞（范围为5 - 70%）。在有明显凋亡变化的区域，Ki-67和半胱天冬酶-3阳性细胞的平均百分比（38.6%）高于无明显凋亡特征的区域（16.3%）。HNL中的一部分细胞，如肿瘤细胞，同时经历增殖和凋亡，从而表现出快速的细胞周期。
