Denis M C, Venne K, Lesiège D, Francoeur M, Groleau S, Guay M, Cusson J, Furtos A
Université de Sherbrooke, Faculté de Médecine et des Sciences de la santé, 3001, 12(e) Avenue Nord, Sherbrooke, Canada J1H 5N4.
J Chromatogr A. 2008 May 2;1189(1-2):410-6. doi: 10.1016/j.chroma.2007.12.061. Epub 2007 Dec 28.
In the present study we evaluated two commonly used iodinated contrast agents, iohexol and iodixanol, as potential markers of impaired renal function. A reversed phase LC-MS method has been developed in order to separate and quantify the two substances. The assay was linear between 0.02 and 9.7 micromol/L for iohexol and between 0.4 and 49.3 micromol/L for iodixanol (r(2) > 0.998). The recovery during sample preparation ranged from 89.1 to 112.4%. The intra- and inter-assay RSD values were 3.06-13.6% for iohexol and 4.32-12.7% for iodixanol. The validated method was subsequently applied to 17 patients scheduled for angiographic procedure displaying normal and impaired renal function. A mixture of iohexol and iodixanol was intra-arterially injected and their corresponding plasma levels were determined periodically over a 24h period following administration. The elimination of both contrast agents followed by the LC-MS approach allowed us to discriminate between patients with normal and impaired renal function at 4, 8 and 24h after administration. Our preliminary results support the predictive value of iohexol and/or iodixanol clearance in a clinical environment to screen and identify patients at risk of developing CIN.
在本研究中，我们评估了两种常用的碘化造影剂——碘海醇和碘克沙醇，作为肾功能受损的潜在标志物。已开发出一种反相液相色谱-质谱法，用于分离和定量这两种物质。该测定法对碘海醇在0.02至9.7微摩尔/升之间呈线性，对碘克沙醇在0.4至49.3微摩尔/升之间呈线性（r²>0.998）。样品制备过程中的回收率在89.1%至112.4%之间。碘海醇的批内和批间相对标准偏差值为3.06 - 13.6%，碘克沙醇为4.32 - 12.7%。随后，将经过验证的方法应用于17名计划进行血管造影术且肾功能正常和受损的患者。动脉内注射碘海醇和碘克沙醇的混合物，并在给药后的24小时内定期测定其相应的血浆水平。通过液相色谱-质谱法对两种造影剂的消除情况进行监测，使我们能够在给药后4小时、8小时和24小时区分肾功能正常和受损的患者。我们的初步结果支持了在临床环境中，碘海醇和/或碘克沙醇清除率对于筛查和识别有发生造影剂肾病风险患者的预测价值。
