Soman Rohit S, Zahir Hamim, Akhlaghi Fatemeh
Clinical Pharmacokinetics Laboratory, Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI 02881, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 25;816(1-2):339-43. doi: 10.1016/j.jchromb.2004.11.046.
An HPLC-UV analytical method for estimation of iohexol in human plasma was developed and validated. Protein precipitation and iohexol extraction from plasma (100 microl) was carried out by adding 800 microl perchloric acid (5%, v/v in water) containing iohexol related compound B as the internal standard followed by vortex mixing and centrifugation. The supernatant (90 microl) was then injected onto a microBondapak C(18) column (150 mm x 3.9 mm, 10 microm) maintained at 30 degrees C. The mobile phase comprised of various proportions of acetonitrile and water with a total run time of 12 min and the wavelength of the UV detector was set at 254 nm. The extraction recovery of iohexol from plasma was >95% and the calibration curve was linear (r(2)=0.99) over iohexol concentrations ranging from 10 to 750 microg/ml (n=8). The method had an accuracy of >92% and intra- and inter-day CV of <3.7% and <3.6%, respectively. The method reported is simple, reliable, precise, accurate and has the capability of being used for determination of iohexol in clinical settings.
建立并验证了一种用于测定人血浆中碘海醇的高效液相色谱 - 紫外分析方法。通过加入800微升含碘海醇相关化合物B作为内标的高氯酸（5%，v/v，以水配制），对100微升血浆进行蛋白质沉淀和碘海醇萃取，随后涡旋混合并离心。取90微升上清液注入到维持在30℃的微Bondapak C(18)柱（150 mm×3.9 mm，10微米）上。流动相由不同比例的乙腈和水组成，总运行时间为12分钟，紫外检测器波长设定为254 nm。碘海醇从血浆中的萃取回收率>95%，在碘海醇浓度为10至750微克/毫升范围内校准曲线呈线性（r(2)=0.99）（n = 8）。该方法的准确度>92%，日内和日间变异系数分别<3.7%和<3.6%。所报道的该方法简单、可靠、精密、准确，有能力用于临床环境中碘海醇的测定。
