Hannink Gerjon, Piek Ester, Hendriks Jose M A, Van der Kraan Peter M, Schreurs B Willem, Buma Pieter
Department of Orthopaedics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
Int Orthop. 2009 Jun;33(3):861-6. doi: 10.1007/s00264-007-0513-8. Epub 2008 Jan 16.
Rinsing bone grafts before or both before and after impaction might have different effects on the incorporation of the graft. Rinsing again after impaction might negatively influence bone induction if growth factors released by impaction are washed away. We studied if transforming growth factor-betas (TGF-betas) and bone morphogenetic proteins (BMPs) are released from the mineralised matrix by impaction and if these released growth factors induce osteogenic differentiation in human mesenchymal stem cells (hMSCs). Rinsed morsellised bone allografts were impacted in a cylinder and the escaping fluid was collected. The fluid was analysed for the presence of TGF-betas and BMPs, and the osteoinductive capacity was tested on hMSCs. Abundant TGF-beta was present in the fluid. No BMPs could be detected. Osteogenic differentiation of hMSCs was inhibited by the fluid. Results from our study leave us only able to speculate whether rinsing grafts again after impaction has a beneficial effect on the incorporation process or not.
在嵌入前或嵌入前后冲洗骨移植物可能会对移植物的整合产生不同影响。如果嵌入释放的生长因子被冲走，嵌入后再次冲洗可能会对骨诱导产生负面影响。我们研究了转化生长因子-β（TGF-β）和骨形态发生蛋白（BMP）是否通过嵌入从矿化基质中释放，以及这些释放的生长因子是否能诱导人间充质干细胞（hMSC）发生成骨分化。将冲洗后的碎骨同种异体移植物嵌入圆柱体中，并收集流出的液体。分析液体中TGF-β和BMP的存在情况，并在hMSC上测试其骨诱导能力。液体中存在大量TGF-β。未检测到BMP。hMSC的成骨分化受到该液体的抑制。我们的研究结果让我们只能推测嵌入后再次冲洗移植物对整合过程是否有有益影响。
