Honeyfield Dale C, Vandergoot Christopher S, Bettoli Phillip W, Hinterkopf Joy P, Zajicek James L
US Geological Survey, Northern Appalachian Research Laboratory, 176 Straight Run Road, Wellsboro, Pennsylvania 16901, USA.
J Aquat Anim Health. 2007 Jun;19(2):84-93. doi: 10.1577/H06-033.1.
We determined the thiamine concentration in egg, muscle, and liver tissues of walleyes Sander vitreus and the fatty acid content of walleye eggs from three southern U.S. reservoirs. In two Tennessee reservoirs (Dale Hollow and Center Hill), in which there were alewives Alosa pseudoharengus in the forage base, natural recruitment of walleyes was not occurring; by contrast in Lake James Reservoir, North Carolina, where there were no alewives, the walleye population was sustained via natural recruitment. Female walleye tissues were collected and assayed for thiamine (vitamin B1) and fatty acid content. Thiamine pyrophosphate was found to be the predominant form of thiamine in walleye eggs. In 2000, mean total egg thiamine concentrations were similar among Center Hill, Dale Hollow, and Lake James reservoirs (2.13, 3.14, and 2.77 nmol thiamine/g, respectively). Egg thiamine concentration increased as maternal muscle (r2 = 0.73) and liver (r2 = 0.68) thiamine concentration increased. Walleye egg thiamine does not appear to be connected to poor natural reproduction in Tennessee walleyes. Threadfin shad Dorosoma petenense, which are found in all three reservoirs, had higher thiaminase activity than alewives. Six fatty acids differed among the walleye eggs for the three reservoirs. Two were physiologically important fatty acids, arachidonic acid (20:4[n-6]) and docosahexaenoic acid (22:6[n-3]), which are important eicosanoid precursors involved in the regulation of biological functions, such as immune response and reproduction.
我们测定了大眼狮鲈（Sander vitreus）的卵、肌肉和肝脏组织中的硫胺素浓度，以及来自美国南部三个水库的大眼狮鲈卵的脂肪酸含量。在田纳西州的两个水库（戴尔空心湖和中央山）中，其饵料基础中有美洲西鲱（Alosa pseudoharengus），大眼狮鲈未出现自然繁殖现象；相比之下，在北卡罗来纳州的詹姆斯湖水库中，没有美洲西鲱，大眼狮鲈种群通过自然繁殖得以维持。采集了雌性大眼狮鲈组织并测定其硫胺素（维生素B1）和脂肪酸含量。发现硫胺素焦磷酸是大眼狮鲈卵中硫胺素的主要形式。2000年，中央山、戴尔空心湖和詹姆斯湖水库的卵中硫胺素平均总浓度相似（分别为2.13、3.14和2.77 nmol硫胺素/克）。卵硫胺素浓度随着母体肌肉（r2 = 0.73）和肝脏（r2 = 0.68）硫胺素浓度的增加而增加。大眼狮鲈卵硫胺素似乎与田纳西州大眼狮鲈自然繁殖不佳无关。在所有三个水库中都能发现的细纹鲱（Dorosoma petenense），其硫胺素酶活性高于美洲西鲱。三个水库的大眼狮鲈卵中的六种脂肪酸存在差异。其中两种是具有生理重要性的脂肪酸，花生四烯酸（20:4[n - 6]）和二十二碳六烯酸（22:6[n - 3]），它们是参与调节生物功能（如免疫反应和繁殖）的重要类二十烷酸前体。
