Skrzeczyńska-Moncznik J, Bzowska M, Loseke S, Grage-Griebenow E, Zembala M, Pryjma J
Department of Immunology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Cracow, Poland.
Scand J Immunol. 2008 Feb;67(2):152-9. doi: 10.1111/j.1365-3083.2007.02051.x.
Based on CD14 and CD16 expression, human peripheral blood monocytes (MO) can be divided into a major CD14(high) CD16(-) population and two minor CD14(high) CD16(+) and CD14(dim) CD16(+) subpopulations. CD14(dim) CD16(+) MO are well characterized and regarded as pro-inflammatory because upon stimulation produce TNF-alpha but little, if any, IL-10. By contrast, little is known about CD14(high) CD16(+) MO. We investigated the surface expression of selected determinants by CD16(+) MO subpopulations, cytokine production, phagocytosis and antigen presentation. We found that both CD16(+) subpopulations had a higher expression of HLA-DR, CD86, CD54 and a lower expression of CD64 than CD14(high) CD16(-) population. In addition, CD14(high) CD16(+) MO showed a higher expression of CD11b and TLR4 than CD14(dim) CD16(+) and CD14(high) CD16(-) subpopulations. CD14(high) CD16(+) MO exhibited an increased phagocytic activity and a decreased antigen presentation in comparison with CD14(dim) CD16(+). As expected, lipopolysaccharide (LPS)-stimulated CD14(dim) CD16(+) MO produced TNF-alpha but little IL-10. By contrast, LPS-stimulated CD14(high) CD16(+) subpopulation produced significantly more IL-10 than CD14(dim) CD16(+) and CD14(high) CD16(-) MO. In conclusion, our data show that human peripheral blood CD16(+) MO are heterogeneous in function and consist of two subpopulations: CD14(dim) CD16(+) pro-inflammatory and CD14(high) CD16(+) with anti-inflammatory potential.
根据CD14和CD16的表达情况，人类外周血单核细胞（MO）可分为一个主要的CD14（高）CD16（-）群体以及两个次要的CD14（高）CD16（+）和CD14（低）CD16（+）亚群。CD14（低）CD16（+）MO具有明确的特征，被认为具有促炎作用，因为受到刺激后会产生肿瘤坏死因子-α（TNF-α），但几乎不产生白细胞介素-10（IL-10），即便有也很少。相比之下，人们对CD14（高）CD16（+）MO了解甚少。我们研究了CD16（+）MO亚群中特定决定簇的表面表达、细胞因子产生、吞噬作用和抗原呈递情况。我们发现，与CD14（高）CD16（-）群体相比，两个CD16（+）亚群的人类白细胞抗原-DR（HLA-DR）、CD86、CD54表达较高，而CD64表达较低。此外，与CD14（低）CD16（+）和CD14（高）CD16（-）亚群相比，CD14（高）CD16（+）MO的CD11b和Toll样受体4（TLR4）表达较高。与CD14（低）CD16（+）相比，CD14（高）CD16（+）MO表现出吞噬活性增强和抗原呈递减少。正如预期的那样，脂多糖（LPS）刺激的CD14（低）CD16（+）MO产生TNF-α，但几乎不产生IL-10。相比之下，LPS刺激的CD14（高）CD16（+）亚群产生的IL-10明显多于CD14（低）CD16（+）和CD14（高）CD16（-）MO。总之，我们的数据表明，人类外周血CD16（+）MO在功能上是异质性的，由两个亚群组成：CD14（低）CD16（+）促炎亚群和具有抗炎潜力的CD14（高）CD16（+）亚群。
