Gao Yuan, Zhang Ming-Chang, Peng Yun
Department of Ophthalmology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Zhonghua Yan Ke Za Zhi. 2007 Oct;43(10):881-4.
To investigate the expression of cyclooxygenase-2 (COX-2) in pterygium and the effect of selective COX-2 inhibitor (Nimesulide) on proliferation of human pterygium fibroblasts (HPF) in culture.
Samples of pterygium and conjunctiva were obtained from patients in the Department of Ophthalmology in Union Hospital of Wuhan. The expression of COX-2 in pterygium was determined by immunohistochemistry. The HPF cells were isolated, cultured and identified. Expression of cyclooxygenase-2 was detected by Western blot in HPF following basic fibroblast growth factor (bFGF) treatment at different concentrations (1, 10, 100 ng/ml). The MTT method was used to assay the biologic activities of Nimesulide in different doses (0, 25, 50, 100, 200 micromol/L) and different time (24, 48, 72, 96 h).
The positive rate of expression of COX-2 was 70%. The expression of COX-2 became more significantly as dose increased with the stimulation of bFGF. Administration of 50 micromol/L Nimesulide for 24 h could significantly inhibit HPF proliferation in a dose- and time-dependent manner (P < 0.05).
bFGF can induce the expression of COX-2 in HPF, COX-2 may be one of factors involved in the formation of pterygium. Nimesulide can significantly inhibit HPF proliferation.
研究环氧化酶-2（COX-2）在翼状胬肉中的表达情况以及选择性COX-2抑制剂（尼美舒利）对体外培养的人翼状胬肉成纤维细胞（HPF）增殖的影响。
从武汉协和医院眼科患者处获取翼状胬肉和结膜样本。采用免疫组织化学法检测翼状胬肉中COX-2的表达。分离、培养并鉴定HPF细胞。用不同浓度（1、10、100 ng/ml）的碱性成纤维细胞生长因子（bFGF）处理HPF后，通过蛋白质免疫印迹法检测COX-2的表达。采用MTT法检测不同剂量（0、25、50、100、200 μmol/L）和不同时间（24、48、72、96 h）的尼美舒利的生物学活性。
COX-2表达阳性率为70%。在bFGF刺激下，COX-2的表达随剂量增加而更显著。给予50 μmol/L尼美舒利24 h可显著抑制HPF增殖，呈剂量和时间依赖性（P < 0.05）。
bFGF可诱导HPF中COX-2的表达，COX-2可能是参与翼状胬肉形成的因素之一。尼美舒利可显著抑制HPF增殖。
