Scuderi Nicolò, Onesti Maria G, Bistoni Giovanni, Ceccarelli Simona, Rotolo Sabrina, Angeloni Antonio, Marchese Cinzia
Department of Plastic and Reconstructive Surgery, University Sapienza, Rome, Italy.
Biomaterials. 2008 Apr;29(11):1620-9. doi: 10.1016/j.biomaterials.2007.12.024. Epub 2008 Jan 16.
The aim of this work was to generate an in vitro skin substitute harbouring autologous fibroblasts, keratinocytes and melanocytes, to establish a new one-step clinical method in problems associated with skin disorders. Here we present a case of a nine-year-old girl with a congenital giant nevus treated by surgical approach, with primary co-cultures of keratinocytes, melanocytes and fibroblasts obtained from autologous skin biopsy. Generally these lesions need to be removed to avoid the risk of transformation into malignant melanoma. With this purpose we analyzed the melanocytes contained in the new skin substitute for the presence of genetic alterations correlated to increased risk for melanoma. The organotypical cultures were designed including an engineered scaffold of a non-woven mesh of hyaluronic acid (HYAFF11). This biomaterial has been previously demonstrated to be the most suitable to maintain polarity and to support the in vitro constructs. Six dermal-epidermal skin substitutes were transplanted and 14 days after surgery the re-epithelialized area was about 90%. Our results suggest that this new dermal-epidermal construct not only reduces hospitalization time and ameliorates scar retraction, but might also represent a solution for the high risk of developing a tumour derived from the original nevus.
这项工作的目的是生成一种含有自体成纤维细胞、角质形成细胞和黑素细胞的体外皮肤替代物，以建立一种针对皮肤疾病相关问题的新的一步临床方法。在此，我们展示了一例采用手术方法治疗的9岁先天性巨痣女孩的病例，其角质形成细胞、黑素细胞和成纤维细胞的原代共培养物取自自体皮肤活检。一般来说，这些病变需要切除以避免转化为恶性黑色素瘤的风险。出于这个目的，我们分析了新皮肤替代物中所含黑素细胞是否存在与黑色素瘤风险增加相关的基因改变。设计了器官型培养，包括一种由透明质酸非织造网（HYAFF11）构成的工程支架。这种生物材料先前已被证明最适合维持极性并支持体外构建体。移植了6个真皮 - 表皮皮肤替代物，术后14天重新上皮化的面积约为90%。我们的结果表明，这种新的真皮 - 表皮构建体不仅减少了住院时间并改善了瘢痕收缩，而且可能也是解决源自原始痣的肿瘤高风险问题的一种方法。
