Gonzalez Ronald, Orchard Ian
Department of Biology, University of Toronto at Mississauga, 3359 Mississauga Road, Mississauga, Ont., Canada L5L 1C6.
Peptides. 2008 Apr;29(4):545-58. doi: 10.1016/j.peptides.2007.11.023. Epub 2007 Dec 14.
The invertebrate neuropeptide Y (NPY) homolog, neuropeptide F (NPF), has been characterized for a wide range of invertebrate phyla, including platyhelminthes, molluscs, and arthropods. Current hypotheses suggest that NPF may be capable of regulating responses to diverse external cues related to nutritional status and feeding. The qualitative and quantitative distribution of an NPF-like peptide in fifth instar Rhodnius prolixus was undertaken using an antiserum raised against Drosophila NPF. Immunohistochemistry reveals NPF-like immunoreactive neurons and processes in the central nervous system, stomatogastric nervous system and peripheral nervous system. The distribution of NPF-like immunoreactivity within the medial neurosecretory cells of the brain and neurohemal areas of the corpus cardiacum and dorsal vessel, suggests NPF may act as a neurohormone. Immunoreactive processes are present over the surface of the hindgut and the immunoreactivity in these processes is greatly reduced in intensity 24h post-feeding. The quantification of partially purified NPF-like material in the CNS of R. prolixus was conducted by HPLC fractionation and radioimmunoassay. The results suggest that NPF-like material is present in fifth instar R. prolixus and likely released into the hemolymph following a blood meal.
无脊椎动物神经肽Y（NPY）的同源物神经肽F（NPF），已在包括扁形动物、软体动物和节肢动物在内的广泛无脊椎动物门类中得到表征。目前的假说表明，NPF可能能够调节对与营养状况和进食相关的各种外部线索的反应。使用针对果蝇NPF产生的抗血清，对五龄红带锥蝽中NPF样肽的定性和定量分布进行了研究。免疫组织化学揭示了中枢神经系统、口胃神经系统和外周神经系统中NPF样免疫反应性神经元和神经突起。大脑内侧神经分泌细胞以及心侧体和背血管的神经血区中NPF样免疫反应性的分布，表明NPF可能作为一种神经激素发挥作用。在后肠表面存在免疫反应性神经突起，并且在进食后24小时，这些神经突起中的免疫反应性强度大大降低。通过高效液相色谱分级分离和放射免疫测定法，对红带锥蝽中枢神经系统中部分纯化的NPF样物质进行了定量分析。结果表明，NPF样物质存在于五龄红带锥蝽中，并且在吸食血液后可能释放到血淋巴中。
