Tsang P W, Orchard I
Department of Zoology, University of Toronto, Ontario, Canada.
J Comp Neurol. 1991 Sep 1;311(1):17-32. doi: 10.1002/cne.903110103.
Immunohistochemistry was used to study the distribution of FMRFamide-like material in the central and peripheral nervous systems and visceral tissues of 5th instar Rhodnius prolixus. Over 200 immunoreactive cell bodies and their processes as well as extensive neuropile regions were distributed throughout the nervous system. Immunoreactive processes were seen over the cephalic aorta, corpus cardiacum/corpus allatum complex, and in neurohaemal sites on the abdominal nerves. In visceral tissues, immunoreactive processes were seen innervating the salivary glands, the foregut, and the hindgut. Immunoreactive cells were also found in the anterior midgut (i.e., the crop and the anterior intestine). A radioimmunoassay specific for "RFamide" carboxy-terminal peptides was used to quantify the amount and the distribution of FMRFamide-like material. Reversed-phase high performance liquid chromatography of nervous tissue extracts revealed several peaks of immunoreactive material. The results suggest the existence of a number of FMRFamide-related peptides in Rhodnius which may have roles in both central and peripheral transmission, may be released as neurohormones and may have endocrine functions in the gut.
免疫组织化学法用于研究五龄红带锥蝽中枢和外周神经系统及内脏组织中类FMRF酰胺物质的分布。200多个免疫反应性细胞体及其突起以及广泛的神经纤维网区域分布于整个神经系统。在头主动脉、心侧体/咽侧体复合体以及腹神经的神经血器官部位可见免疫反应性突起。在内脏组织中，可见免疫反应性突起支配唾液腺、前肠和后肠。在前肠中部（即嗉囊和前肠）也发现了免疫反应性细胞。使用针对“RFamide”羧基末端肽的放射免疫分析法来定量类FMRF酰胺物质的含量和分布。神经组织提取物的反相高效液相色谱显示出几个免疫反应性物质峰。结果表明，红带锥蝽中存在多种与FMRF酰胺相关的肽，它们可能在中枢和外周传递中发挥作用，可能作为神经激素释放，并且可能在肠道中具有内分泌功能。
