Tataurov Andrey V, You Yong, Owczarzy Richard
Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA.
Biophys Chem. 2008 Mar;133(1-3):66-70. doi: 10.1016/j.bpc.2007.12.004. Epub 2007 Dec 23.
Synthetic oligodeoxynucleotides are widely used in many biological, biochemical and biophysical applications. The concentration, composition and structure of DNA are often determined from its ultraviolet spectrum. Although parameters for use with the nearest-neighbor model for prediction of extinction coefficients of single stranded DNAs at 260 nm were published some time ago, similar parameters for other wavelengths or for use with DNA duplexes have not been reported. Practical formulae and parameters for prediction of UV spectra, hypochromism and peak wavelengths were experimentally determined for both single stranded and double stranded oligodeoxynucleotides in the range from 215 to 310 nm. The accuracy of predictions made using the nearest-neighbor model and the base composition model was determined and compared. The spectrum of any DNA oligomer can be calculated using a Microsoft Excel application that is available in the Appendix A.
合成寡脱氧核苷酸广泛应用于许多生物学、生物化学和生物物理学领域。DNA的浓度、组成和结构通常由其紫外光谱确定。尽管用于预测单链DNA在260nm处消光系数的最近邻模型参数已于一段时间前公布，但尚未报道其他波长或用于DNA双链体的类似参数。通过实验确定了单链和双链寡脱氧核苷酸在215至310nm范围内紫外光谱、减色效应和峰值波长预测的实用公式和参数。确定并比较了使用最近邻模型和碱基组成模型进行预测的准确性。任何DNA寡聚物的光谱都可以使用附录A中提供的Microsoft Excel应用程序进行计算。
