Kallansrud G, Ward B
Sigma Biosciences, Sigma Chemical Company, St. Louis, Missouri, 63178, USA.
Anal Biochem. 1996 Apr 5;236(1):134-8. doi: 10.1006/abio.1996.0141.
The hyperchromicity resulting from exhaustive hydrolysis of several single-stranded and double-stranded oligodeoxynucleotides (ODN) with snake venom phosphodiesterase or snake venom phosphodiesterase/DNase I was used to measure the ODN's extinction coefficient at 260 nm. These extinction coefficients were compared with those obtained using nonempirical approximations. For the oligodeoxynucleotides investigated, the nonempirical approximations yielded extinction coefficients within 20% of the measured values.
用蛇毒磷酸二酯酶或蛇毒磷酸二酯酶/脱氧核糖核酸酶I对几种单链和双链寡脱氧核苷酸（ODN）进行彻底水解，由此产生的增色效应被用于测定ODN在260nm处的消光系数。这些消光系数与使用非经验近似法获得的消光系数进行了比较。对于所研究的寡脱氧核苷酸，非经验近似法得出的消光系数在测量值的20%以内。
