用于平行 G-四链体的荧光猝灭配体。

Fluorescence Turn-Off Ligand for Parallel G-Quadruplexes.

机构信息

Department of Bioanalytical Chemistry, Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland.

Laboratory of Molecular Assays and Imaging, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland.

出版信息

Molecules. 2024 Aug 18;29(16):3907. doi: 10.3390/molecules29163907.

DOI:10.3390/molecules29163907

PMID:39202986

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11357100/

Abstract

Parallel-stranded G-quadruplex structures are found to be common in the human promoter sequences. We tested highly fluorescent 9-methoxyluminarine ligand (9-MeLM) binding interactions with different parallel G-quadruplexes DNA by spectroscopic methods such as fluorescence and circular dichroism (CD) titration as well as UV melting profiles. The results showed that the studied 9-MeLM ligand interacted with the intramolecular parallel G-quadruplexes (G4s) with similar affinity. The binding constants of 9-methoxyluminarine with different parallel G4s were determined. The studies upon oligonucleotides with different flanking sequences on c- G-quadruplex suggest that 9-methoxyluminarine may preferentially interact with 3'end of the c- promoter. The high decrease in 9-MeLM ligand fluorescence upon binding to all tested G4s indicates that 9-methoxyluminarine molecule can be used as a selective fluorescence turn-off probe for parallel G-quadruplexes.

摘要

平行股 G-四链体结构在人类启动子序列中很常见。我们通过荧光和圆二色性（CD）滴定以及紫外熔融曲线等光谱方法测试了高荧光 9-甲氧基呫吨（9-MeLM）配体与不同平行 G-四链体 DNA 的结合相互作用。结果表明，研究的 9-MeLM 配体与分子内平行 G-四链体（G4s）具有相似的亲和力。确定了 9-甲氧基呫吨与不同平行 G4 的结合常数。对具有不同侧翼序列的寡核苷酸的研究表明，9-甲氧基呫吨可能优先与 c-启动子的 3'端相互作用。结合所有测试的 G4 后，9-MeLM 配体荧光的高降低表明 9-甲氧基呫吨分子可以用作平行 G-四链体的选择性荧光关闭探针。