Kim Jong-Seo, Kim Ji-Hyun, Kim Hie-Joon
Department of Chemistry, Seoul National University, Seoul, Korea 151-747.
Rapid Commun Mass Spectrom. 2008;22(4):495-502. doi: 10.1002/rcm.3392.
Picolinamidination of amino groups in peptides was carried out using ethyl picolinimidate tetrafluoroborate synthesized from picolinamide and triethyloxonium tetrafluoroborate. The N-terminal amino group as well as the epsilon-amino group of lysine were derivatized. The matrix-assisted laser desorption/ionization (MALDI) signal of a peptide was enhanced 20-35-fold upon picolinamidination depending on the number of amino groups derivatized. The signal enhancement effect is much higher than that of acetamidination or guanidination previously reported. Improved protein identification by mass mapping of the derivatized peptides was demonstrated.
使用由吡啶甲酰胺和四氟硼酸三乙氧基鎓合成的四氟硼酸乙酯吡啶亚胺对肽中的氨基进行吡啶甲酰胺化。N 端氨基以及赖氨酸的 ε-氨基都被衍生化。根据衍生化氨基的数量，吡啶甲酰胺化后肽的基质辅助激光解吸/电离（MALDI）信号增强了 20 - 35 倍。该信号增强效果远高于先前报道的乙酰酰胺化或胍基化。通过衍生化肽的质量图谱证明了蛋白质鉴定的改进。
