Hambourger Michael, Gervaldo Miguel, Svedruzic Drazenka, King Paul W, Gust Devens, Ghirardi Maria, Moore Ana L, Moore Thomas A
Center for Bioenergy and Photosynthesis and Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
J Am Chem Soc. 2008 Feb 13;130(6):2015-22. doi: 10.1021/ja077691k. Epub 2008 Jan 19.
The Clostridium acetobutylicum [FeFe]-hydrogenase HydA has been investigated as a hydrogen production catalyst in a photoelectrochemical biofuel cell. Hydrogenase was adsorbed to pyrolytic graphite edge and carbon felt electrodes. Cyclic voltammograms of the immobilized hydrogenase films reveal cathodic proton reduction and anodic hydrogen oxidation, with a catalytic bias toward hydrogen evolution. When corrected for the electrochemically active surface area, the cathodic current densities are similar for both carbon electrodes, and approximately 40% of those obtained with a platinum electrode. The high surface area carbon felt/hydrogenase electrode was subsequently used as the cathode in a photoelectrochemical biofuel cell. Under illumination, this device is able to oxidize a biofuel substrate and reduce protons to hydrogen. Similar photocurrents and hydrogen production rates were observed in the photoelectrochemical biofuel cell using either hydrogenase or platinum cathodes.
丙酮丁醇梭菌[FeFe]氢化酶HydA已作为光电化学生物燃料电池中的产氢催化剂进行了研究。氢化酶吸附在热解石墨边缘和碳毡电极上。固定化氢化酶膜的循环伏安图显示了阴极质子还原和阳极氢氧化，具有向析氢的催化偏向。当校正电化学活性表面积时，两种碳电极的阴极电流密度相似，约为铂电极所获电流密度的40%。高表面积碳毡/氢化酶电极随后被用作光电化学生物燃料电池的阴极。在光照下，该装置能够氧化生物燃料底物并将质子还原为氢气。在使用氢化酶或铂阴极的光电化学生物燃料电池中观察到了相似的光电流和产氢速率。
