Zha Jinmiao, Sun Liwei, Spear Philip A, Wang Zijian
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, PR China.
Ecotoxicol Environ Saf. 2008 Oct;71(2):390-9. doi: 10.1016/j.ecoenv.2007.11.017. Epub 2008 Feb 21.
Breeding pairs of rare minnows were exposed to 5, 10, 20 microg/L 4-nonylphenol (NP) or 4 ng/L 17alpha-ethinylestradiol (EE(2)) for 21 days. Among reproductive parameters investigated, fecundity, fertility, and laying interval were significantly affected by 4 ng/L EE(2), but were not affected at any exposure level of NP. These effects coincided with male feminization, ova-testis, increased plasma VTG in both males and females as well as the accumulation of eosinophilic material in kidneys and livers. Histopathologic evaluation revealed severe kidney lesions in both EE(2) and NP-exposed fish. The bioassay succeeded in demonstrating that EE(2) was several orders of magnitude more potent than the industrial chemical NP. The rare minnow was more sensitive to EE(2) than medaka, but comparable to zebrafish in terms of sensitivity. The results of the present investigation indicated that a short-term reproductive bioassay is feasible using the Chinese rare minnow as the test species.
将稀有鲦鱼的繁殖配对暴露于5、10、20微克/升的4-壬基酚（NP）或4纳克/升的17α-乙炔雌二醇（EE(2)）中21天。在所研究的生殖参数中，4纳克/升的EE(2)对产卵量、受精率和产卵间隔有显著影响，但在任何NP暴露水平下均无影响。这些影响与雄性雌性化、卵睾、雌雄两性血浆中VTG增加以及肾脏和肝脏中嗜酸性物质的积累相一致。组织病理学评估显示，暴露于EE(2)和NP的鱼类均有严重的肾脏病变。生物测定成功地证明，EE(2)的效力比工业化学品NP高几个数量级。稀有鲦鱼对EE(2)的敏感性高于青鳉，但在敏感性方面与斑马鱼相当。本研究结果表明，以中国稀有鲦鱼作为试验物种进行短期生殖生物测定是可行的。
