Hamurcu Zuhal, Dönmez-Altuntas Hamiyet, Patiroglu Türkan
The College of Physical Education and Sports, Erciyes University, 38039 Kayseri, Turkey.
Cancer Genet Cytogenet. 2008 Jan 15;180(2):140-4. doi: 10.1016/j.cancergencyto.2007.10.009.
Structural chromosomal aberrations have been described in various types of human leukemia. The micronucleus technique provides a measure of both chromosome breakage and chromosome loss. The present study investigated micronucleus (MN) frequency in mitogen-stimulated peripheral blood lymphocytes from 20 newly diagnosed and untreated leukemia patients: 4 with acute lymphoblastic leukemia (ALL), 10 with acute myeloid leukemia (AML), and 6 with chronic lymphocytic leukemia (CLL). The mean MN frequency for untreated patients was 3.65 +/- 1.47 in ALL, 3.55 +/- 1.24 in AML, 3.03 +/- 1.05 in CLL. No differences in MN frequency were seen between leukemia types ALL, AML, and CLL (P = 0.503). The mean basal MN frequency for all patients, regardless of leukemia type, was 3.41 +/- 1.19, which was significantly higher (P = 0.001) than that of 20 age-matched control subjects, 1.87 +/- 0.75. Although no significant relationship was found between age and MN frequency in patients with leukemia (r = 0.050; P = 0.835), the MN frequency in the lymphocytes of healthy control increased regularly and significantly with age (r = 0.531; P = 0.016). These data indicate that the increased baseline MN frequency in lymphocytes of untreated patients with leukemia may reflect genomic instability or deficiency of DNA repair capacity. MN enhancement in this disease may thus be a consequence of the disease process.
在各类人类白血病中均已发现染色体结构畸变。微核技术可用于衡量染色体断裂和染色体丢失情况。本研究调查了20例新诊断且未经治疗的白血病患者经丝裂原刺激的外周血淋巴细胞中的微核（MN）频率：4例急性淋巴细胞白血病（ALL）患者、10例急性髓系白血病（AML）患者和6例慢性淋巴细胞白血病（CLL）患者。未经治疗患者的平均MN频率在ALL中为3.65±1.47，在AML中为3.55±1.24，在CLL中为3.03±1.05。ALL、AML和CLL这几种白血病类型之间的MN频率未见差异（P = 0.503）。所有患者（无论白血病类型）的平均基础MN频率为3.41±1.19，显著高于20名年龄匹配的对照受试者的频率1.87±0.75（P = 0.001）。虽然白血病患者的年龄与MN频率之间未发现显著相关性（r = 0.050；P = 0.835），但健康对照者淋巴细胞中的MN频率随年龄增长而有规律且显著增加（r = 0.531；P = 0.016）。这些数据表明，未经治疗的白血病患者淋巴细胞中基线MN频率的增加可能反映了基因组不稳定性或DNA修复能力的缺陷。因此，这种疾病中MN频率的升高可能是疾病进程的结果。
