Dimitrijević A, Milosević-Djordjević O, Grujicić D, Arsenijević S
Department of Obstetrics and Gynecology of Clinic of Kragujevac, Serbia and Montenegro.
Mutat Res. 2006 Sep 19;608(1):43-8. doi: 10.1016/j.mrgentox.2006.05.002. Epub 2006 Jun 13.
The main aim of the present study was to investigate the influence of infection with the intracellular bacterium Chlamydia trachomatis, and subsequent treatments with oral doxycycline or azithromycin on the frequency of micronuclei (MN) in peripheral blood lymphocytes of adult female patients receiving standard doses of these drugs. The frequency of micronuclei was measured in the lymphocytes of 38 newly diagnosed adult women with genital C. trachomatis infection. Samples were taken before and after the therapy, and from 50 healthy control females. The therapy was taken orally during 10 days at 2 x 100 mg per day, and then for another 10 days at 1 x 100 mg per day for doxycycline, and as a single dose of 1g for azithromycin. Isolated lymphocytes from all subjects were cultured by use of the whole-blood method and blocked in metaphase with cytochalasin B (Cyt B). One thousand binucleate cells per subject were scored according to published criteria. The frequency of micronuclei was not significantly higher in samples of infected females before therapy, compared with the baseline frequency in healthy control females (p > 0.05). In patients who received doxycycline, the micronucleus frequency after the end of therapy was significantly higher than before treatment (p < 0.001). The mean frequency of micronuclei in females after the end of the therapy with azithromycin did not show an increase (p > 0.05). The application of linear regression analysis showed that the difference in micronucleus frequency before and after therapy (effect of the antibiotics) was affected by the therapy type. Age and smoking did not affect micronucleus frequency in analyzed samples of patients (p = 0.078, 0.579). We conclude that C. trachomatis infection does not induce micronuclei in peripheral blood lymphocytes of infected adult female patients. Therapy with doxycycline significantly increases the micronucleus frequency in lymphocytes of treated patients, but treatment with azithromycin does not induce micronuclei.
本研究的主要目的是调查细胞内细菌沙眼衣原体感染，以及随后口服强力霉素或阿奇霉素治疗，对接受标准剂量这些药物的成年女性患者外周血淋巴细胞微核（MN）频率的影响。在38例新诊断为生殖器沙眼衣原体感染的成年女性淋巴细胞中测量微核频率。在治疗前后以及50名健康对照女性中采集样本。强力霉素治疗为每天2次，每次100mg，持续10天，然后每天1次，每次100mg，再持续10天；阿奇霉素为单次剂量1g。使用全血法培养所有受试者分离出的淋巴细胞，并用细胞松弛素B（Cyt B）将其阻断在中期。根据已发表的标准，对每个受试者的1000个双核细胞进行评分。与健康对照女性的基线频率相比，感染女性治疗前样本中的微核频率没有显著更高（p>0.05）。接受强力霉素治疗的患者，治疗结束后的微核频率显著高于治疗前（p<0.001）。阿奇霉素治疗结束后女性的微核平均频率没有增加（p>0.05）。线性回归分析表明，治疗前后微核频率的差异（抗生素的作用）受治疗类型影响。年龄和吸烟对分析的患者样本中的微核频率没有影响（p = 0.078，0.579）。我们得出结论，沙眼衣原体感染不会在受感染成年女性患者的外周血淋巴细胞中诱导微核形成。强力霉素治疗显著增加了治疗患者淋巴细胞中的微核频率，但阿奇霉素治疗不会诱导微核形成。
