Teixeira Ana Claudia, Dos Santos Raquel Alves, Poersch Aline, Carrara Helio Humberto Angotti, de Andrade Jurandyr Moreira, Takahashi Catarina Satie
Int J Clin Exp Med. 2009 Oct 20;2(3):280-8.
The present study evaluated the basal DNA damage and the cellular response to this damage induced by in vitro administration of Etoposide in lymphocytes donated by twenty untreated breast cancer (BC) patients and twenty age-matched healthy women. Micronucleus (MN) and alkaline Comet assays were performed in cultured peripheral blood lymphocytes (PBL) according to a standard protocol for in vitro treatment with various concentrations of Etoposide or a control. For the Comet Assay, three samples of cells were collected: T(0) (immediately preceding treatment of the cultures), T(1) (immediately after completion of the treatment) and T(2) (four hours after completion of the treatment). MN frequency in the BC group treated with 25 muM Etoposide (19.1 +/- 7.35) was significantly higher than the control (10.9 +/- 9.87) group. In the alkaline Comet Assay, both the BC group and the healthy women showed the ability to repair Etoposide-induced DNA damage within 4 hours of reincubation.
本研究评估了20名未经治疗的乳腺癌（BC）患者和20名年龄匹配的健康女性捐献的淋巴细胞中，依托泊苷体外给药诱导的基础DNA损伤及其细胞对该损伤的反应。根据用不同浓度依托泊苷或对照进行体外处理的标准方案，在培养的外周血淋巴细胞（PBL）中进行微核（MN）和碱性彗星试验。对于彗星试验，收集三个细胞样本：T(0)（培养物处理前即刻）、T(1)（处理完成后即刻）和T(2)（处理完成后4小时）。用25μM依托泊苷处理的BC组的MN频率（19.1±7.35）显著高于对照组（10.9±9.87）。在碱性彗星试验中，BC组和健康女性均显示在再孵育4小时内有修复依托泊苷诱导的DNA损伤的能力。
