[Phagocytic activity of neutrophils (PMN cells) in cattle infected with IBR/IPV virus (bovine herpesvirus 1--BHV1)].

PMN cell random and directed migration, adherence, aggregation, ingestion of bacteria as well as resting and stimulated NBT reduction activities were studied in two groups of 5, 2-4 months-old bullocks experimentally (group I) or naturally (group III) infected with BHV1. Control groups (II and IV) consisted of 5 healthy calves. Experimental infection of bullous were carried out using Polish field isolate of BHV1; animals were injected intramuscularly, intravenously and into conjunctival sack with 3 ml, 1 ml and 0.5 ml of the virus respectively. PMN cell phagocytic activities and virological examinations were performed in the group I and II control 2 days before, at the day, and at 1, 2, 3, 4, 7, 14, 21, 28, 35, 42, 64 days post infection, while in the groups III and IV at 1, 7, 14, 21, 28, 42 and 52 day of the experiment. Directed migration, ingestion of bacteria and NBT reduction by resting and stimulated PMN cells activities were decreased whereas random migration, adherence and aggregation abilities as well as number phagocytosing cells were increased both in naturally and experimentally infected animals. These changes were noted in experimentally infected animals at 1-7 day post infection, its three weeks before antibody anti BHV1 and lasted for 3-9 weeks, while in naturally infected bullocks they appeared at 1-28 day of the experiment and persisted for 4-8 weeks the virus was easy isolated from nasal swabs especially of naturally infected animals.