Liu C S, Zhang J, Zhou T H
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Yao Xue Xue Bao. 1991;26(9):682-7.
A GC-MS method for the investigation on the metabolism of dehydrochloromethyltestosterone (DHCMT) in man is described. Steroids in human urine were adsorbed on a macroporous XAD-2 resin, eluted with methanol, hydrolyzed with glucuronidase, extracted and concentrated for derivatization and then analyzed with GC-MS. DHCMT and its 7 metabolites were detected in urine samples 8 to 30 h after oral administration. Their structures and the variation of their concentration in urine were determined and the possible metabolic pathways of DHCMT were proposed on the basis of GC-MS feature of its metabolites. The specific metabolites and characteristic ions for screening DHCMT positive urine were chosen. Furthermore, the influence of the sample pretreatment methods on GC-MS results has been studied.
本文描述了一种用于研究脱氢氯甲基睾酮（DHCMT）在人体中代谢情况的气相色谱-质谱联用（GC-MS）方法。人尿中的类固醇被吸附在大孔XAD-2树脂上，用甲醇洗脱，用葡萄糖醛酸酶水解，提取并浓缩用于衍生化，然后用GC-MS进行分析。口服给药后8至30小时的尿液样本中检测到了DHCMT及其7种代谢物。根据代谢物的GC-MS特征确定了它们的结构及其在尿液中的浓度变化，并提出了DHCMT可能的代谢途径。选择了用于筛查DHCMT阳性尿液的特定代谢物和特征离子。此外，还研究了样品预处理方法对GC-MS结果的影响。
