Wu Jia, Deng Xiao, Tian Binqiang, Wang Lan, Xie Bijun
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
J Agric Food Chem. 2008 Feb 13;56(3):1131-7. doi: 10.1021/jf072727d. Epub 2008 Jan 23.
This paper describes the binding of Calcofluor, a fluorescent probe, to oat beta-glucan in buffer solutions. The binding equilibrium constant (K), the total number of binding sites per beta-glucan molecule (N), and the average binding number of Calcofluor per beta-glucan molecule (n) were determined by UV spectroscopic method. The results indicate that the association of Calcofluor and beta-glucan is driven by both enthalpy and entropy and that the process involves hydrogen bonding, van der Waals forces, and hydrophobic interaction. Higher buffer concentration and NaCl facilitate the binding of Calcofluor to beta-glucan. The adsorption isotherm fits a Langmuir model quite well.
本文描述了荧光探针荧光增白剂在缓冲溶液中与燕麦β-葡聚糖的结合情况。通过紫外光谱法测定了结合平衡常数（K）、每个β-葡聚糖分子的结合位点总数（N）以及每个β-葡聚糖分子荧光增白剂的平均结合数（n）。结果表明，荧光增白剂与β-葡聚糖的缔合受焓和熵的驱动，该过程涉及氢键、范德华力和疏水相互作用。较高的缓冲液浓度和氯化钠促进荧光增白剂与β-葡聚糖的结合。吸附等温线与朗缪尔模型拟合得很好。
