Nakayama Koji, Tachikawa Takashi, Majima Tetsuro
The Institute of Scientific and Industrial Research (ISIR), Osaka University Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.
Langmuir. 2008 Mar 4;24(5):1625-8. doi: 10.1021/la703354c. Epub 2008 Jan 23.
Protein patterning on solid surfaces is a topic of significant importance in the fields of biosensors, diagnostic assays, cell adhesion technologies, and biochip microarrays. In this letter, we have established a novel, rapid method for the fabrication of a "protein recording material", which enables us to spatiotemporally regulate the recording, reading, and erasing of a fluorescent protein array as information by a photochemical technique. A photolinker that we synthesized here was used to control the protein array spatiotemporally. The recording process was almost completed after 1 min of photoirradiation to read a clear pattern consisting of a specific protein-ligand complex with high spatiotemporal resolution. The erasing of the protein array was then achieved by photoirradiation onto the entire patterned surface.
固体表面上的蛋白质图案化是生物传感器、诊断检测、细胞粘附技术和生物芯片微阵列领域中极为重要的一个课题。在本信函中，我们建立了一种新颖、快速的方法来制备“蛋白质记录材料”，该方法使我们能够通过光化学技术对荧光蛋白阵列的记录、读取和擦除进行时空调控，以此作为信息。我们在此合成的一种光交联剂被用于对蛋白质阵列进行时空控制。光照射1分钟后，记录过程基本完成，从而能够以高时空分辨率读取由特定蛋白质 - 配体复合物组成的清晰图案。然后通过对整个图案化表面进行光照射来实现蛋白质阵列的擦除。
