Deepak Praveen, Kumar Sanjay, Acharya Arbind
Immunology Laboratory, Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi, UP, India.
Am J Reprod Immunol. 2008 Feb;59(2):127-38. doi: 10.1111/j.1600-0897.2007.00541.x.
Dalton's lymphoma (DL) shows very high interleukin (IL)-13 level in the serum and ascitic fluid. Therefore, in the present study, we investigated if any sexual dichotomy in the level of IL-13, and resulting B-cell activation and Ig subclass switching also exist in the tumor-bearing host.
Serum and ascitic fluid of different groups of DL-bearing mice were isolated and IL-13 level and various Ig levels in serum were quantified by double-sandwich enzyme-linked immunosorbent assay. Further, the B cells were isolated from DL-bearing mice and effects of various concentrations of IL-13 and sex steroids were measured.
Uncastrated and hormone replaced DL-bearing mice showed sexual dichotomy in IL-13 level, and subsequent difference in Ig-level and this was found to be more pronounced in females. Similarly, in vitro study suggested that estrogen treatment, in combination with IL-13, strongly modulates B-cell Ig switching in comparison to testosterone treatment in association with IL-13.
It can be concluded that IL-13, in concert with gonadal hormones, differentially modulates the B-cell function in a tumor-bearing host.
