Oddi S, Fezza F, Pasquariello N, De Simone C, Rapino C, Dainese E, Finazzi-Agrò A, Maccarrone M
Department of Biomedical Sciences, University of Teramo, Teramo, Italy.
Cell Mol Life Sci. 2008 Mar;65(5):840-50. doi: 10.1007/s00018-008-7494-7.
Anandamide is a lipid messenger that carries out a wide variety of biological functions. It has been suggested that anandamide accumulation involves binding to a saturable cellular component. To identify the structure(s) involved in this process, we analyzed the intracellular distribution of both biotinylated and radiolabeled anandamide, providing direct evidence that lipid droplets, also known as adiposomes, constitute a dynamic reservoir for the sequestration of anandamide. In addition, confocal microscopy and biochemical studies revealed that the anandamide-hydrolase is also spatially associated with lipid droplets, and that cells with a larger adiposome compartment have an enhanced catabolism of anandamide. Overall, these findings suggest that adiposomes may have a critical role in accumulating anandamide, possibly by connecting plasma membrane to internal organelles along the metabolic route of this endocannabinoid.
花生四烯乙醇胺是一种执行多种生物学功能的脂质信使。有人提出，花生四烯乙醇胺的积累涉及与一种可饱和的细胞成分结合。为了确定参与这一过程的结构，我们分析了生物素化和放射性标记的花生四烯乙醇胺的细胞内分布，提供了直接证据表明脂滴（也称为脂肪体）构成了隔离花生四烯乙醇胺的动态储存库。此外，共聚焦显微镜和生化研究表明，花生四烯乙醇胺水解酶在空间上也与脂滴相关，并且具有较大脂肪体区室的细胞对花生四烯乙醇胺的分解代谢增强。总体而言，这些发现表明脂肪体可能在积累花生四烯乙醇胺方面具有关键作用，可能是通过沿着这种内源性大麻素的代谢途径将质膜与内部细胞器连接起来。
