Kopera Ilona, Tuz Ryszard, Kotula-Balak Malgorzata, Schwarz Tomasz, Koczanowski Jozef, Bilinska Barbara
Department of Endocrinology and Tissue Culture, Institute of Zoology, Jagiellonian University, Kraków, Poland.
J Exp Zool A Ecol Genet Physiol. 2008 Mar 1;309(3):117-26. doi: 10.1002/jez.437.
In this study we thoroughly scrutinized testes morphology and investigated whether treatment of recipient boars with gonadotropin-releasing hormone (GnRH)-agonist deslorelin could alter the expression of 3beta-hydroxysteroid dehydrogenase (3beta-HSD), luteinizing hormone receptors (LHRs), and androgen receptors (ARs) in testicular cells. An implant containing 4.7 mg of the GnRH-agonist deslorelin was subcutaneously inserted into crossbred male pigs at 91 and 147 days of age. Testicular traits, morphology of the testes, the proteins' expression, and testosterone concentration in blood plasma were analyzed in all boars after slaughter at 175 days of age. Histological analysis revealed significant alterations in both the interstitial tissue and seminiferous tubules of experimental animals after 28 and 84 days of deslorelin treatment. The intensity of the AR immunostaining within the testis appeared as a function of the severity of testicular dysgenesis. Time-dependent action of deslorelin on the expression of LHR and 3beta-HSD in Leydig cells was also detected. Staining for LHR and 3beta-HSD was very weak or the Leydig cells were immunonegative. Concomitantly, a significant decrease in plasma testosterone level was found in both groups of deslorelin-treated boars when compared with the control group. This is the first report showing the cellular distribution of AR, LHR, and 3beta-HSD in testicular cells of deslorelin-treated boars. It is concluded that morphological and immunohistochemical studies are important for the evaluation of testicular histoarchitecture and steroidogenic function. Subsequently, the endocrine control of reproduction in the GnRH-agonist deslorelin-treated males will be better understood.
在本研究中，我们全面仔细地检查了睾丸形态，并研究了用促性腺激素释放激素（GnRH）激动剂地洛瑞林处理受体公猪是否会改变睾丸细胞中3β-羟基类固醇脱氢酶（3β-HSD）、促黄体生成素受体（LHR）和雄激素受体（AR）的表达。在91日龄和147日龄时，将含有4.7 mg GnRH激动剂地洛瑞林的植入物皮下插入杂种公猪体内。在175日龄屠宰所有公猪后，分析其睾丸特征、睾丸形态、蛋白质表达和血浆睾酮浓度。组织学分析显示，地洛瑞林处理28天和84天后，实验动物的间质组织和生精小管均出现显著变化。睾丸内AR免疫染色的强度表现为睾丸发育不全严重程度的函数。还检测到地洛瑞林对睾丸间质细胞中LHR和3β-HSD表达的时间依赖性作用。LHR和3β-HSD染色非常弱或睾丸间质细胞免疫阴性。同时，与对照组相比，两组接受地洛瑞林处理的公猪血浆睾酮水平均显著降低。这是首次报道地洛瑞林处理公猪睾丸细胞中AR、LHR和3β-HSD的细胞分布。结论是形态学和免疫组织化学研究对于评估睾丸组织结构和类固醇生成功能很重要。随后，将更好地理解GnRH激动剂地洛瑞林处理的雄性动物的生殖内分泌控制。
