Koyasu Toshiyuki, Kondo Mineo, Miyata Kentaro, Ueno Shinji, Miyata Takaki, Nishizawa Yuji, Terasaki Hiroko
Department of Ophthalmology, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya, Japan.
Curr Eye Res. 2008 Jan;33(1):91-9. doi: 10.1080/02713680701823232.
To study the properties of the photopic electroretinograms (ERGs) of the metabotropic glutamate receptor subtype 6 (mGluR6)-deficient mice and to investigate the contribution of cone ON-and OFF-pathways to the mouse photopic ERGs.
Photopic ERGs were recorded from mGluR6-deficient and wild-type mice. Photopic ERGs were also recorded after an intravitreous injection of cis-2,3 piperidine dicarboxylic acid (PDA) to block the transmission of signals from the photoreceptors to the OFF-bipolar cells, horizontal cells, and other inner retinal neurons.
The amplitude of the b-wave of the photopic ERG was severely reduced in mGluR6-deficient mice, but a small, slow, positive component was seen after the a-wave. Intravitreous injection of PDA eliminated this positive component.
The mGluR6-deficient mouse is a useful animal model to study the contribution of the ON-and OFF-pathways to the mouse ERG.
研究代谢型谷氨酸受体6（mGluR6）缺陷小鼠明视觉视网膜电图（ERG）的特性，并探讨视锥细胞ON通路和OFF通路对小鼠明视觉ERG的作用。
记录mGluR6缺陷小鼠和野生型小鼠的明视觉ERG。在玻璃体内注射顺式-2,3-哌啶二羧酸（PDA）以阻断从光感受器到OFF双极细胞、水平细胞和其他视网膜内层神经元的信号传递后，也记录明视觉ERG。
mGluR6缺陷小鼠明视觉ERG的b波振幅严重降低，但在a波之后可见一个小的、缓慢的正向成分。玻璃体内注射PDA消除了这个正向成分。
mGluR6缺陷小鼠是研究ON通路和OFF通路对小鼠ERG作用的有用动物模型。
